Ann Lab Med 2017; 37(2): 147-150  
Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Blood
Hee Jae Huh, M.D.1, Jong Eun Park, M.D.1, Ji-Youn Kim, M.T.2, Sun Ae Yun, M.T.2, Myoung-Keun Lee, M.T.1, Nam Yong Lee, M.D.1, Jong-Won Kim, M.D.1, and Chang-Seok Ki, M.D.1
Department of Laboratory Medicine and Genetics1, Center for Clinical Medicine2, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea
Correspondence to: Chang-Seok Ki
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: +82-2-3410-2709 Fax: +82-2-3410-2719 E-mail: changski@skku.edu
Received: August 13, 2016; Revised: September 18, 2016; Accepted: December 5, 2016; Published online: March 1, 2017.
© The Korean Society for Laboratory Medicine. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB. We used 81 WB clinical specimens to compare performance of the Real-Q EBV Quantification Kit and artus EBV RG PCR Kit (Qiagen, Germany). The limit of detection (LOD) and limit of quantification (LOQ) for the Real-Q kit were 453 and 750 IU/mL, respectively. The conversion factor from EBV genomic copies to IU was 0.62. The linear range of the assay was from 750 to 106 IU/mL. Viral load values measured with the Real-Q assay were on average 0.54 log10 copies/mL higher than those measured with the artus assay. The Real-Q assay offered good analytical performance for EBV DNA quantification in WB.
Keywords: Epstein-Barr virus, Real-time PCR, Quantification, Whole blood, Performance


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