Ann Lab Med 2017; 37(2): 151-154
Dengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015
Muhammad Suleman, M.Phil.1,2, Rani Faryal, Ph.D.1, Muhammad Masroor Alam, Ph.D.2, Salmaan Sharif, Ph.D.2, Shahzad Shaukat, Ph.D.2, Uzma Bashir Aamir, M.Phil.2, Adnan Khurshid, M.Phil.2, Mehar Angez, M.Phil.2, Massab Umair, M.Phil.2, Mian Muhammad Sufian, M.S.2, Yasir Arshad, M.S.2, and Syed Sohail Zahoor Zaidi, M.Phil.2
Department of Microbiology1, Quaid-i-Azam University, Islamabad; Department of Virology2, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan
Correspondence to: Syed Sohail Zahoor Zaidi
Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad-45500, Pakistan
Tel: +92-519255147 E-mail:
Received: July 21, 2016; Revised: October 10, 2016; Accepted: December 1, 2016; Published online: March 1, 2017.
© The Korean Society for Laboratory Medicine. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period. Our findings suggest the need for an active surveillance system coupled with the laboratory diagnosis, especially in the chronic endemic areas of the country. Public awareness programs are needed for effective control and prevention of outbreaks in the future.
Keywords: Dengue, Pakistan, Epidemiology, Real-time RT-PCR, Serotypes

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