Ann Lab Med 2017; 37(3): 272-276  
Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution
Eun Hye Cho, M.D.1,*, Kyung Sun Park, M.D.2,*, Mina Yang, M.D.1, Dong Joon Song, M.T.1, Hee Jae Huh, M.D.1, Chang-Seok Ki, M.D.1, and Nam Yong Lee, M.D.1
Department of Laboratory Medicine and Genetics1, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul; SD Genomics Co., Ltd.2, Seoul, Korea
Correspondence to: Hee Jae Huh
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: +82-2-3410-1836
Fax: +82-2-3410-2719
E-mail: pmhhj77@gmail.com
Nam Yong Lee
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: +82-2-3410-2706
Fax: +82-2-3410-2719
E-mail: micro.lee@samsung.com
*These authors contributed equally to this work.
Received: July 22, 2016; Revised: October 4, 2016; Accepted: December 29, 2016; Published online: May 1, 2017.
© The Korean Society for Laboratory Medicine. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing.
Keywords: Leptotrichia, Bacteremia, Identification, MALDI-TOF MS, 16S ribosomal RNA


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