Ann Lab Med 2018; 38(1): 23-31
Comparison of Singleplex Specific IgE Detection Immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy
Kyung Hee Park, M.D.1,2*, Jongsun Lee, M.S.2, Da Woon Sim, M.D.1,3, and Sang Chul Lee, M.D.1,2
Division of Allergy and Immunology1, Department of Internal Medicine, Yonsei University College of Medicine, Seoul; Institute of Allergy2, Yonsei University College of Medicine, Seoul; Division of Allergy3, Asthma, and Clinical Immunology, Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea
Corresponding author: Kyung Hee Park
Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea
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Received: March 13, 2017; Revised: May 17, 2017; Accepted: September 15, 2017; Published online: January 1, 2018.
© Korean Society for Laboratory Medicine. All rights reserved.

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Background: The identification of clinically meaningful specific immunoglobulin E (sIgE) is important for the diagnosis and management of allergic diseases. Various in vitro sIgE detection methods are available worldwide. Depending on the number of antigens that can be tested simultaneously, there are two representative methods: singleplex and multiplex. Singleplex sIgE detection is mainly provided by Thermo Fisher (ImmunoCAP) and Siemens (Immulite). This study aimed to compare the diagnostic agreement of two singleplex sIgE detection assays.
Methods: Sera from 209 Korean patients with allergic disease were used to compare the ImmunoCAP and Immulite assays with respect to the following allergens: inhalant allergens (Dermatophagoides farinae, cat and dog dander, oak, rye grass, mugwort, Alternaria, German cockroach) and food allergens (hen’s egg white, cow’s milk, wheat, peanut, soybean, and shrimp). Data from 902 paired comparison tests were included for comparisons. Qualitative, semi-quantitative, and quantitative comparisons were performed using statistical analyses.
Results: In qualitative comparisons, the positivity and negativity agreements ranged from 75% (wheat, shrimp) to 96% (Alternaria). Class consistency (classes 0–6) was well matched. Spearman’s rank correlation coefficients for all allergens except shrimp were over 0.7. In quantitative comparisons, all allergens excluding shrimp showed 〉0.7 intra-class correlation coefficients.
Conclusions: The ImmunoCAP and Immulite systems showed similar performances. However, clinicians should consider fundamental methodological differences between the assays.
Keywords: Allergens, Immunoassay, Immunoglobulin E, Comparison, Agreement

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