Ann Lab Med 2018; 38(4): 362-366  https://doi.org/10.3343/alm.2018.38.4.362
Flow-Cytometric Analysis of HNA-2 Expression and Phenotypes Among Thai Blood Donors
Oytip Nathalang, Ph.D.1, Kanokpol Siriphanthong, B.S.1, Sawang Petvises, Ph.D.2, and Nichapa Jeumjanya, M.S.3
Graduate Program in Biomedical Sciences1 and Department of Medical Technology2, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand; Blood Bank Section3, Thammasat University Hospital, Pathumtani, Thailand
Corresponding author: Oytip Nathalang,
Graduate Program, Faculty of Allied Health Sciences, Thammasat University, 99 Moo 18 Klongluang, Pathumtani 12120, Thailand
Tel: +66-29869213 Ext. 7231
Fax: +66-25165379
E-mail: oytipntl@hotmail.com
Received: June 5, 2017; Revised: August 1, 2017; Accepted: February 1, 2018; Published online: July 1, 2018.
© Korean Society for Laboratory Medicine. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Antibodies specific to human neutrophil antigen (HNA), especially HNA-2, are implicated in various conditions, including neonatal alloimmune neutropenia, febrile non-hemolytic transfusion reactions, and transfusion-related acute lung injury. The distribution of the HNA-2 phenotype frequencies in the Thai population remains unknown. This study aimed to investigate HNA-2 phenotype frequencies in Thai blood donors and to compare the relationships of sex and age with HNA-2 expression.
Methods: EDTA blood samples were collected from 220 unrelated healthy Thai blood donors, including 150 males and 70 females, with ages ranging from 20 to 57 years. Polymorphonuclear cells were isolated and stained with monoclonal antibodies clone MEM-166 and clone 2D1, which are specific to human CD177 (HNA-2) and CD45, respectively. HNA-2 expression according to sex and age was analyzed by flow cytometry.
Results: Among the 220 donors, HNA-2-positive and HNA-2-null-phenotype frequencies were 0.995 and 0.005, respectively. Mean antigen expression was significantly higher in women (71.01±15.46%) than in men (64.59±18.85%; P <0.05). No significant differences in HNA-2 expression were found between different age groups. HNA-2 phenotype frequencies were similar to those in Asian, African, American, and Brazilian populations, but were significantly different from those in eastern Japanese, Korean, and French populations (P <0.001).
Conclusions: This is the first report of HNA-2 phenotype frequencies in a Thai population, and the data will be helpful in predicting the risk of HNA-2 alloimmunization and in recruiting granulocyte panel donors.
Keywords: HNA-2, Phenotype frequencies, Flow cytometry, Thais



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