Ann Lab Med 2020; 40(1): 1-6  https://doi.org/10.3343/alm.2020.40.1.1
JL1 Antigen Expression on Bone Marrow Lymphoma Cells from Patients With Non-Hodgkin Lymphoma
Min-Sun Kim, M.D.1, Chan-Jeoung Park, M.D., Ph.D.1, Young-Uk Cho, M.D., Ph.D.1, Seongsoo Jang, M.D., Ph.D.1, Eul-Ju Seo, M.D., Ph.D.1, Chan-Sik Park, M.D., Ph.D.2, Jooryung Huh, M.D., Ph.D.2, Ho Joon Im, M.D., Ph.D.3, Jong Jin Seo, M.D., Ph.D.3, Dok Hyun Yoon, M.D., Ph.D.4, and Cheolwon Suh, M.D., Ph.D.4
Departments of 1Laboratory Medicine, 2Pathology, 3Pediatrics, and 4Oncology, Asan Medical Center and University of Ulsan College of Medicine, Seoul, Korea
Corresponding author: Chan-Jeoung Park, M.D., Ph.D.
University of Ulsan College of Medicine and Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
Tel: +82-2-3010-4508 Fax: +82-2-478-0884 E-mail: cjpark@amc.seoul.kr
Received: December 4, 2018; Revised: March 16, 2019; Accepted: July 26, 2019; Published online: January 1, 2020.
© Korean Society for Laboratory Medicine. All rights reserved.

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Abstract
Background: JL1, a CD43 epitope and mucin family cell surface glycoprotein, is expressed on leukemic cells. An anti-JL1 antibody combined with a toxic substance can have targeted therapeutic effects against JL1-positive leukemia; however, JL1 expression on bone marrow (BM) lymphoma cells has not been assessed using flow cytometry. We investigated JL1 expression on BM lymphoma cells from patients with non-Hodgkin lymphoma (NHL) to assess the potential of JL1 as a therapeutic target.
Methods: Patients with BM involvement of mature B-cell (N=44) or T- and natural killer (NK)-cell (N=4) lymphomas were enrolled from May 2015 to September 2016. JL1 expression on BM lymphoma cells was investigated using flow cytometry. Clinical, pathological, and cytogenetic characteristics, and treatment responses were compared according to JL1 expression status.
Results: Of the patients with NHL and BM involvement, 37.5% (18/48) were JL1-positive. Among mature B-cell lymphomas, 100%, 38.9%, 33.3%, 100%, and 25.0% of Burkitt lymphomas, diffuse large B-cell leukemias, mantle cell leukemias, Waldenstrom macroglobulinemia, and other B-cell lymphomas, respectively, were JL1-positive. Three mature T- and NK-cell NHLs were JL1-positive. JL1 expression was associated with age (P=0.045), complete response (P=0.004), and BM involvement at follow-up (P=0.017), but not with sex, performance status, the B symptoms, packed marrow pattern, cytogenetic abnormalities, or survival.
Conclusions: JL1 positivity was associated with superior complete response and less BM involvement in NHL following chemotherapy.
Keywords: JL1 expression, Flow cytometry, Mature B-cell lymphoma, T- and natural killer-cell lymphoma



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