Ann Lab Med 2020; 40(4): 317-320  https://doi.org/10.3343/alm.2020.40.4.317
Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus
In Young Yoo, M.D., Ph.D.1, Ji-Youn Kim, M.T.2, Young Kyung Yoon, M.D., Ph.D.3,4, Hee Jae Huh, M.D., Ph.D.1, and Nam Yong Lee, M.D., Ph.D.1
1Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea; 2Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea; 3Division of Infectious Diseases, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea; 4Institute of Emerging Infectious Diseases, Korea University College of Medicine, Seoul, Korea
Corresponding author: Hee Jae Huh, M.D.
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: +82-2-3410-1836 Fax: +82-2-3410-2719. E-mail: pmhhj77@gmail.com
Received: September 6, 2019; Revised: November 17, 2019; Accepted: January 15, 2020; Published online: July 1, 2020.
© Korean Society for Laboratory Medicine. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed Huaiyangshan banyangvirus). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a- chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV.
Keywords: Huaiyangshan banyangvirus, SFTS virus, Performance, Turnaround time, SFTS-QS kit, PowerChek SFTSV Real-time PCR kit



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