Genetic distance tree and plasmid types of penicillinase-producing Neisseria gonorrhoeae with TEM-1 or TEM-135 grouped according to porB genotype. Alleles marked with a black circle or numbers shown in red are associated with isolates harboring TEM-135; numbers in parenthesis indicate the number of isolates with the same sequence type. Although sequence types are diverse among isolates with TEM-135, two small clusters (cluster A and B) accounting for 56.3% (9/16) of all TEM-135 isolates appear to be independently segregated.