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Fig. 2.

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Fig. 2.

Effects of LINC01234 knockdown on CRC cell viability and apoptosis in vitro. (A) and (B) LINC01234 expression level was determined by qRT-PCR following transfection of SW480 and HCT116 cells with si-LINC01234 (N=6) or si-NC. *P<0.001 vs Scrambled. (C) and (D) CCK-8 analyses were used to determine the viability of si-LINC01234-transfected SW480 and HCT116 cells. Values are from average of three independent experiments. P<0.01 vs Scrambled. (E) Colony-forming analyses were conducted to determine the proliferation of si-LINC01234-transfected SW480 and HCT116 cells. Values are from three independent experiments. *P<0.001 vs Scrambled. (F) Flow cytometry was performed to analyze apoptosis rates in CRC cells transfected with si-LINC01234 after 48 hours. Values are from three independent experiments. *P<0.001, P<0.01 vs Scrambled. (G) Western blot analysis of apoptosis-related proteins after transfection of SW480 and HCT116 cells with Scrambled siRNA, si-LINC01234 1#, or si-LINC01234 2#. GAPDH was used as an internal control. Data are presented as mean±SD.

Abbreviations: CRC, colorectal cancer; qRT-PCR, quantitative reverse transcription PCR; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CCK-8, Cell counting Kit 8.

Ann Lab Med 2020;40:155~163 https://doi.org/10.3343/alm.2020.40.2.155

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