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Fig. 4.

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Fig. 4.

LINC01234 represses KLF6 expression. (A) KLF6 expression in CRC tissues (N=76) compared with that in corresponding non-tumor tissues (N=76). KLF6 expression was examined by qRT-PCR and normalized to GAPDH expression. *P<0.01, NC vs CRC. (B) Co-expression analysis between LINC01234 and KLF6. (C) KLF6 mRNA levels were detected by qRT-PCR following the transfection of SW480 and HCT116 cells with si-LINC01234; the results were normalized to GAPDH expression. P<0.001 vs Scrambled. (D) & (E) KLF6 protein levels were determined by western blotting following the transfection of SW480 and HCT116 cells with si-LINC01234. Values are from three independent experiments. P<0.001 vs Scrambled. (F) KLF6 expression level in mouse tumors was determined by qRT-PCR formed from SW480/sh-LINC01234 or SW480/empty vector (N=8). P<0.001, sh-LINC01234 vs Empty vector. Data are presented as mean±SD.

Abbreviations: KLF6, Krüppel-like factor 6; qRT-PCR, quantitative reverse transcription PCR; CRC, colorectal cancer; NC, negative control (adjacent non-tumor tissues); GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Ann Lab Med 2020;40:155~163

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