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Fig. 5.

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Fig. 5.

Overexpression of KLF6 inhibits CRC cell proliferation and increases apoptosis. (A) mRNA level of KLF6 in SW480 and HCT116 cells transfected with pCMV-Tag2B-KLF6 was measured by qRT-PCR. (B)–(D) CCK-8 and colony-forming analyses were used to determine the viability of pCMV-Tag2B-KLF6-transfected SW480 and HCT116 cells. Values are from three independent experiments. (E) Apoptosis was determined by flow cytometry. Values are from three independent experiments. Data are presented as mean±SD. *P<0.001, P<0.01.

Abbreviations: KLF6, Krüppel-like factor 6; CRC, colorectal cancer; CCK-8, Cell Counting Kit 8; qRT-PCR, quantitative reverse transcription PCR.

Ann Lab Med 2020;40:155~163 https://doi.org/10.3343/alm.2020.40.2.155

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