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Table. 1.

Table. 1.

Gradient conditions for chromatographic separation for DNA-TG quantification

Time segment Time (min) Flow rate (μL/min) Mobile phase
%A* %B
1 Initial 0.35 0 100
2 1.5 0.35 0 100
3 1.8 0.45 0 100
4 2.7 0.45 0 100
5 3.2 0.45 70 30
6 3.7 0.45 70 30
7 3.9 0.45 0 100

*0.1% formic acid in distilled water; 160 mM formic acid and 10 mM ammonium formate in 95% acetonitrile.

Abbreviation: DNA-TG, DNA-incorporated 6-thioguanine.

Ann Lab Med 2021;41:145~154 https://doi.org/10.3343/alm.2021.41.2.145

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