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Table. 1.

Table. 1.

Clinical filamentous fungi isolates identified using the VITEK MS v3.0 system in comparison with sequence-based identification

Sequence-based identification (N of isolates) N (%) of isolates at initial testing N (%) of isolates at additive testing


Correct ID Incomplete ID Mis-ID No ID Correct ID Incomplete ID Mis-ID No ID
Aspergillus species (37)
Aspergillus flavus/oryzae (9) 7 (77.8) 0 0 2 (22.2) 9 (100) 0 0 0
Aspergillus fumigatus (8) 6 (75.0) 0 0 2 (25.0) 7 (87.5) 0 0 1 (12.5)
Aspergillus niger (6) 3 (50.0) 1 (16.7) 0 2 (33.3) 5 (83.3) 0 0 1 (16.7)
Aspergillus terreus (4) 4 (100) 0 0 0 4 (100) 0 0 0
Aspergillus sydowii (3) 2 (66.7) 0 0 1 (33.3) 3 (100) 0 0 0
Aspergillus tubingensis (3)* 1 (33.3) 0 0 2 (66.7) 3 (100) 0 0 0
Aspergillus nidulans (2) 1 (50.0) 0 0 1 (50.0) 2 (100) 0 0 0
Aspergillus lentulus (1) 0 0 0 1 (100) 1 (100) 0 0 0
Aspergillus westerdijkiae (1)* 1 (100) 0 0 0 1 (100) 0 0 0
Subtotal (37) 25 (67.6) 1 (2.7) 0 11 (29.7) 35 (94.6) 0 0 2 (5.4)
Dermatophytes (41)
Trichophyton rubrum (12) 8 (66.7) 1 (8.3) 0 3 (25.0) 10 (83.3) 0 1 (8.3) 1 (8.3)
Trichophyton interdigitale (11) 7 (63.6) 0 0 4 (36.4) 9 (81.8) 0 0 2 (18.2)
Trichophyton tonsurans (3) 0 0 0 3 (100) 3 (100) 0 0 0
Trichophyton verrucosum (3) 1 (33.3) 0 0 2 (66.7) 1 (33.3) 1 (33.3) 0 1 (33.3)
Microsporum canis (5) 4 (80.0) 0 0 1 (20.0) 5 (100) 0 0 0
Microsporum gypseum (4) 1 (25.0) 0 1 (25.0) 2 (50.0) 1 (25.0) 0 1 (25.0) 2 (50.0)
Epidermophyton floccosum (3) 2 (66.7) 0 0 1 (33.3) 3 (100) 0 0 0
Subtotal (41) 23 (56.1) 1 (2.4) 1 (2.4) 16 (39.0) 32 (78.0) 1 (2.4) 2 (4.9) 6 (14.6)
Other molds (27)
Penicillium citrinum (5) 1 (20.0) 0 0 4 (80.0) 1 (20.0) 0 0 4 (80.0)
Penicillium camemberti (1) 0 0 0 1 (100) 0 0 0 1 (100)
Penicillium chrysogenum (1) 1 (100) 0 0 0 1 (100) 0 0 0
Penicillium expansum (1) 0 0 0 1 (100) 0 0 0 1 (100)
Fusarium solani (4) 3 (75.0) 0 0 1 (25.0) 4 (100) 0 0 0
Fusarium proliferatum (1) 1 (100) 0 0 0 1 (100) 0 0 0
Alternaria alternata (3) 3 (100) 0 0 0 3 (100) 0 0 0
Alternaria astragali (1)* 0 0 0 1 (100) 0 1 (100) 0 0
Scedosporium apiospermum (2) 1 (50.0) 0 0 1 (50.0) 2 (100) 0 0 0
Scedosporium boydii (1) 0 0 0 1 (100) 0 0 0 1 (100)
Cladosporium cladosporioides (1) 0 0 0 1 (100) 0 0 0 1 (100)
Cladosporium sphaerospermum (1)* 0 0 0 1 (100) 0 0 0 1 (100)
Acremonium sclerotigenum (1) 1 (100) 0 0 0 1 (100) 0 0 0
Cunninghamella bertholletiae (1)* 0 0 0 1 (100) 0 0 0 1 (100)
Lichtheimia corymbifera (1) 0 0 0 1 (100) 0 0 0 1 (100)
Paecilomyces variotii (1) 1 (100) 0 0 0 1 (100) 0 0 0
Purpureocillium lilacinum (1) 1 (100) 0 0 0 1 (100) 0 0 0
Subtotal
Only database (25) 13 (52.0) 0 0 12 (48.0) 15 (60.0) 0 0 10 (40.0)
All species (27) 13 (48.1) 0 0 14 (51.9) 15 (55.6) 1 (3.7) 0 11 (40.7)
Total molds (105)
Only database (103) 61 (59.2) 2 (1.9) 1 (1.0) 39 (37.9) 82 (79.6) 1 (1.0) 2 (1.9) 18 (17.5)
All species (105) 61 (58.1) 2 (1.9) 1 (1.0) 41 (39.0) 82 (78.1) 2 (1.9) 2 (1.9) 19 (18.1)

*Aspergillus tubingensis, A. westerdijkiae, and Cladosporium sphaerospermum were not included in the v3.0 database but were included in the v3.2 database, whereas Alternaria astragali and Cunninghamella bertholletiae were not included in either database. A. tubingensis, A. westerdijkiae, and C. sphaerospermum were identified using the VITEK MS v3.2 system; Isolates with an initial testing result of “no identification” or “multiple species identification” were retested following the same VITEK MS method. The additive testing results included the retested results, as well as the initial results of all acceptable identifications; One isolate of Microsporum gypseum was misidentified as Epidermophyton floccosum.

Abbreviations: ID, identification; Mis-ID, misidentification.

Ann Lab Med 2021;41:214~220 https://doi.org/10.3343/alm.2021.41.2.214

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