Table. 2. Table 2

Clinical filamentous fungi isolates that were retested using the VITEK MS v3.0 system

Sequence-based identification (N of isolates)		Initial testing		Retesting^†

		Culture medium	Incubation time (days)	ID results	Culture medium	Incubation time (days)
Aspergillus species (12)
	Aspergillus flavus/oryzae	SDA	5	Aspergillus flavus	SDA	6
	Aspergillus flavus/oryzae	SDA	7	Aspergillus flavus	SDA	3
	Aspergillus fumigatus	PDA	13	Aspergillus fumigatus	SDA	3
	Aspergillus fumigatus	PDA	2	No ID	PDA	4
	Aspergillus niger^‡	SDA	14	Aspergillus niger complex	SDA	4
	Aspergillus niger	SDA	5	Aspergillus niger complex	SDA	2
	Aspergillus niger	PDA	2	No ID	PDA	4
	Aspergillus sydowii	PDA	7	Aspergillus sydowii	PDA	18
	Aspergillus tubingensis^*	PDA	5	Aspergillus niger complex	PDA	5
	Aspergillus tubingensis^*	PDA	7	Aspergillus niger complex	PDA	5
	Aspergillus nidulans	PDA	3	Aspergillus nidulans	PDA	4
	Aspergillus lentulus	PDA	8	Aspergillus lentulus	PDA	6
Dermatophytes (17)
	Trichophyton rubrum^‡	SDA	9	No ID	SDA	13
	Trichophyton rubrum	PDA	13	Trichophyton rubrum	PDA	17
	Trichophyton rubrum	PDA	16	Trichophyton rubrum	PDA	12
	Trichophyton rubrum	SDA	9	Fusarium proliferatum	SDA	13
	Trichophyton interdigitale	PDA	10	Trichophyton interdigitale	PDA	6
	Trichophyton interdigitale	PDA	20	Trichophyton interdigitale	SDA	12
	Trichophyton interdigitale	PDA	16	No ID	PDA	12
	Trichophyton interdigitale	PDA	17	No ID	PDA	12
	Trichophyton tonsurans	SDA	9	Trichophyton tonsurans	SDA	13
	Trichophyton tonsurans	SDA	9	Trichophyton tonsurans	SDA	13
	Trichophyton tonsurans	SDA	9	Trichophyton tonsurans	SDA	20
	Trichophyton verrucosum	PDA	14	Trichophyton interdigitale	PDA	8
	Trichophyton verrucosum	PDA	14	No ID	PDA	12
	Microsporum canis	SDA	9	Microsporum canis	SDA	23
	Microsporum gypseum	SDA	9	No ID	SDA	13
	Microsporum gypseum	SDA	9	No ID	SDA	13
	Epidermophyton floccosum	SDA	13	Epidermophyton floccosum	SDA	14
Other molds (14)
	Penicillium citrinum	PDA	10	No ID	PDA	8
	Penicillium citrinum	PDA	10	No ID	PDA	8
	Penicillium citrinum	PDA	10	No ID	PDA	8
	Penicillium citrinum	PDA	13	No ID	PDA	8
	Penicillium camemberti	PDA	3	No ID	PDA	14
	Penicillium expansum	PDA	14	No ID	PDA	14
	Fusarium solani	SDA	5	Fusarium solani complex	SDA	6
	Alternaria astragali^*	PDA	4	Alternaria alternata	PDA	4
	Scedosporium apiospermum	SDA	5	Scedosporium apiospermum	SDA	6
	Scedosporium boydii	PDA	10	No ID	PDA	8
	Cladosporium cladosporioides	PDA	7	No ID	PDA	14
	Cladosporium sphaerospermum^*	PDA	16	No ID	PDA	5
	Cunninghamella bertholletiae^*	PDA	2	No ID	PDA	4
	Lichtheimia corymbifera	PDA	6	No ID	PDA	4
Total (43)

^*Aspergillus tubingensis and Cladosporium sphaerospermum were not included in the v3.0 database but were included in the v3.2 database, whereas Alternaria astragali and Cunninghamella bertholletiae were not included in either database. A. tubingensis and C. sphaerospermum were identified using the VITEK MS v3.2 system; ^†Isolates with an initial testing result of “no identification” or “multiple species identification” were retested following the same VITEK MS method; ^‡All except these two isolates showed “no identification” results at the initial testing. The Aspergillus niger isolate showed “multiple species identification” result as Candida haemulonii, Aspergillus niger complex, Candida rugosa, and Candida parapsilosis. The Trichophyton rubrum isolate showed “multiple species identification” result as Trichophyton rubrum and Trichophyton violaceum.

Abbreviations: ID, identification; PDA, potato dextrose agar; SDA, Sabouraud dextrose agar.

Ann Lab Med 2021;41:214~220 https://doi.org/10.3343/alm.2021.41.2.214