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Table. 2.

Table. 2.

Clinical filamentous fungi isolates that were retested using the VITEK MS v3.0 system

Sequence-based identification (N of isolates) Initial testing Retesting


Culture medium Incubation time (days) ID results Culture medium Incubation time (days)
Aspergillus species (12)
Aspergillus flavus/oryzae SDA 5 Aspergillus flavus SDA 6
Aspergillus flavus/oryzae SDA 7 Aspergillus flavus SDA 3
Aspergillus fumigatus PDA 13 Aspergillus fumigatus SDA 3
Aspergillus fumigatus PDA 2 No ID PDA 4
Aspergillus niger SDA 14 Aspergillus niger complex SDA 4
Aspergillus niger SDA 5 Aspergillus niger complex SDA 2
Aspergillus niger PDA 2 No ID PDA 4
Aspergillus sydowii PDA 7 Aspergillus sydowii PDA 18
Aspergillus tubingensis* PDA 5 Aspergillus niger complex PDA 5
Aspergillus tubingensis* PDA 7 Aspergillus niger complex PDA 5
Aspergillus nidulans PDA 3 Aspergillus nidulans PDA 4
Aspergillus lentulus PDA 8 Aspergillus lentulus PDA 6
Dermatophytes (17)
Trichophyton rubrum SDA 9 No ID SDA 13
Trichophyton rubrum PDA 13 Trichophyton rubrum PDA 17
Trichophyton rubrum PDA 16 Trichophyton rubrum PDA 12
Trichophyton rubrum SDA 9 Fusarium proliferatum SDA 13
Trichophyton interdigitale PDA 10 Trichophyton interdigitale PDA 6
Trichophyton interdigitale PDA 20 Trichophyton interdigitale SDA 12
Trichophyton interdigitale PDA 16 No ID PDA 12
Trichophyton interdigitale PDA 17 No ID PDA 12
Trichophyton tonsurans SDA 9 Trichophyton tonsurans SDA 13
Trichophyton tonsurans SDA 9 Trichophyton tonsurans SDA 13
Trichophyton tonsurans SDA 9 Trichophyton tonsurans SDA 20
Trichophyton verrucosum PDA 14 Trichophyton interdigitale PDA 8
Trichophyton verrucosum PDA 14 No ID PDA 12
Microsporum canis SDA 9 Microsporum canis SDA 23
Microsporum gypseum SDA 9 No ID SDA 13
Microsporum gypseum SDA 9 No ID SDA 13
Epidermophyton floccosum SDA 13 Epidermophyton floccosum SDA 14
Other molds (14)
Penicillium citrinum PDA 10 No ID PDA 8
Penicillium citrinum PDA 10 No ID PDA 8
Penicillium citrinum PDA 10 No ID PDA 8
Penicillium citrinum PDA 13 No ID PDA 8
Penicillium camemberti PDA 3 No ID PDA 14
Penicillium expansum PDA 14 No ID PDA 14
Fusarium solani SDA 5 Fusarium solani complex SDA 6
Alternaria astragali* PDA 4 Alternaria alternata PDA 4
Scedosporium apiospermum SDA 5 Scedosporium apiospermum SDA 6
Scedosporium boydii PDA 10 No ID PDA 8
Cladosporium cladosporioides PDA 7 No ID PDA 14
Cladosporium sphaerospermum* PDA 16 No ID PDA 5
Cunninghamella bertholletiae* PDA 2 No ID PDA 4
Lichtheimia corymbifera PDA 6 No ID PDA 4
Total (43)

*Aspergillus tubingensis and Cladosporium sphaerospermum were not included in the v3.0 database but were included in the v3.2 database, whereas Alternaria astragali and Cunninghamella bertholletiae were not included in either database. A. tubingensis and C. sphaerospermum were identified using the VITEK MS v3.2 system; Isolates with an initial testing result of “no identification” or “multiple species identification” were retested following the same VITEK MS method; All except these two isolates showed “no identification” results at the initial testing. The Aspergillus niger isolate showed “multiple species identification” result as Candida haemulonii, Aspergillus niger complex, Candida rugosa, and Candida parapsilosis. The Trichophyton rubrum isolate showed “multiple species identification” result as Trichophyton rubrum and Trichophyton violaceum.

Abbreviations: ID, identification; PDA, potato dextrose agar; SDA, Sabouraud dextrose agar.

Ann Lab Med 2021;41:214~220 https://doi.org/10.3343/alm.2021.41.2.214

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