pISSN 2234-3806eISSN 2234-3814
Drug measurement methods used in participating laboratories
| Laboratory code | Test drugs | Internal standard | LC manufacturer* | MS instrument manufacturer* | MS instrument model |
|---|---|---|---|---|---|
| A | TAC+SIR+EVE | Ascomycin+SIR-d3+EVE-d4 | Shimadzu | SCIEX | Triple Quad 4500 |
| CSA | CSD | Agilent | Agilent | 6460 Triple Quadrupole | |
| MPA | Indomethacin | Waters | Waters | Quattro Micro | |
| B | TAC+SIR+EVE+CSA | Ascomycin | Waters | Waters | Quattro Premier |
| C | TAC+SIR+EVE | Ascomycin+SIR-d3+EVE-d4 | Waters | Waters | Xevo TQ-S |
| D | TAC+CSA | Ascomycin+CSD | Waters | Waters | Xevo TQD |
| MPA | Indomethacin | Waters | Waters | Xevo TQD | |
| E | TAC+SIR+EVE | Ascomycin | Waters | Waters | Xevo TQD |
| CSA | CSD | Waters | Waters | Xevo TQD | |
| F | TAC+SIR+EVE+CSA | Ascomycin+EVE-d4+CSD | Agilent | Agilent | 6490 Triple Quadrupole |
| G | TAC+SIR+EVE+CSA | Ascomycin+SIR-d3+EVE-d4+CSD | Agilent | SCIEX | API 4000 |
| H | TAC+SIR+EVE+CSA | TAC-13C,d2+SIR-d3+EVE-d4+CSA-d4 | Agilent | SCIEX | API 3200 |
| I | TAC+SIR+EVE+CSA | Ascomycin | Agilent | SCIEX | QTRAP 5500 |
| MPA | MPA-d3 | Agilent | SCIEX | QTRAP 5500 | |
| J | TAC+SIR+EVE+CSA | Ascomycin+CSD | Agilent | SCIEX | Triple Quad 3500 |
Most laboratories used precipitation with organic solvent mixture followed by centrifugation for extraction, except Lab I, which used liquid/liquid extraction. All the participating laboratories used the commercial calibrator from Chromsystems Instruments & Chemicals GmbH (Gräfelfing, Germany).
*The manufacturer names are indicated using their short names: Agilent, Agilent Technologies (Santa Clara, CA, USA); SCIEX, AB SCIEX (Foster City, CA,USA); Shimadzu, Shimadzu Corporation (Kyoto, Japan); Waters, Waters Corporation (Milford, MA, USA).
Abbreviations: CSA, cyclosporine; CSD, cyclosporine D; EVE, everolimus; LC, liquid chromatography; MPA, mycophenolic acid; MS, mass spectrometry;TAC, tacrolimus; SIR, sirolimus.
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