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Table. 1.

Table. 1.

Donor screening through clinical assessment and laboratory tests

Stage 1. Clinical assessment Stage 2. Laboratory tests

Stool tests Serological screening Additional tests
General questions - Date of birth - Gender (pregnant if female) - Height, weight - Logistics*Questions associated with infectious/transmissible diseases - High-risk sexual behavior - Recent travel history to high-risk countries - Infectious disease risk (piercing, tattoos, imprisonment) - Any relationship with a person with a transmittable diseaseGI-related questions - Recent GI symptoms or disease - Atopic syndrome, asthma, allergies - Autoimmune disease - Chronic pain - Mental health condition (depression, ADHD/ADD, anxiety) - Neurological disease (e.g., Alzheimer’s, Parkinson’s) - Medical history (surgery, malignancy, other diagnoses) - Restrictive diet or eating disorder - Family history (GI disorder, colon cancer) - Medication or supplement use within three months (e.g., antibiotic, antifungal, antiviral, prescription medicine, herbal medicine) Viral tests - Viruses associated with diarrhea (RT-PCR): Rotavirus, Norovirus GI/GII, Adenovirus, AstrovirusParasitic tests - Parasites and ova (multiplex PCR): Ascaris lumbricoides, Cryptosporidium parvum/hominis, Ancylostoma duodenale, Necator americanus, Strongyloides stercoralis, Giardia lamblia, Entamoeba histolytica, Trichuris trichiura, Clonorchis sinensis, Diphyllobothrium latum, Blastocystis hominisBacterial tests - Helicobacter pylori (nested PCR) - Bacteria associated with diarrhea (PCR): Salmonella spp., Shigella spp., Vibrio spp. Campylobacter spp. (C. jejuni, C. coli), Yersinia enterocolitica, Aeromonas spp. - Multidrug-resistant bacteria: MRSA (Cx), CRE (Cx + PCR), VRE (Cx + PCR), ESBL-producing Enterobacteriaceae (Cx)Additional fecal tests - Fecal white blood cell - Occult blood Viral tests - Hepatitis A virus IgM - Hepatitis B virus surface antigen - Hepatitis C virus antibody - HIV I, II - Epstein-Barr virus (IgG, IgM) - Cytomegalovirus (IgG, IgM)Parasitic test: Not applicableBacterial test - Syphilis reagin testOther blood tests - Routine chemistry - Amylase/lipase - C-reactive protein - LDL/HDL cholesterol - Triglyceride - Antinuclear antibody test - Erythrocyte sedimentation rate - Insulin - Complete blood count - Platelet count - Urea breath test§- Chest (posteroanterior) radiography

*To determine the rate of possible donation and donor capability to deliver fecal material within two hrs. Regions at high risk of (1) communicable disease/traveler’s diarrhea (e.g., Southeast Asia, Africa, and the Middle East) and Creutzfeldt-Jakob disease (United Kingdom, Europe). Routine chemistry tests include tests for calcium, inorganic phosphate, glucose, blood urea nitrogen, creatinine, uric acid, total cholesterol, total protein, albumin, alkaline phosphatase, aspartate transaminase, alanine aminotransferase, serum glutamate-pyruvate transaminase, and total bilirubin. §Added in May 2019 for more rigorous screening for asymptomatic Helicobacter pylori carriers.

Abbreviations: GI, gastrointestinal; ADHD/ADD, attention deficit hyperactivity disorder/attention deficit disorder; RT-PCR, reverse transcriptase-polymerase chain reaction; MRSA, methicillin-resistant Staphylococcus aureus; Cx, culture; CRE, carbapenem-resistant Enterobacteriaceae; VRE, vancomycin-resistant enterococci; ESBL, extended spectrum β-lactamase; LDL, low density lipoprotein; HDL, high density lipoprotein.

Ann Lab Med 2021;41:424~428 https://doi.org/10.3343/alm.2021.41.4.424

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