OPEN ACCESS pISSN 2234-3806
eISSN 2234-3814

Table. 1.

Table. 1.

Categorical agreement between fluconazole susceptibility results determined by the BMD and DDT for 188 Candida isolates with known and unknown molecular mechanisms

Species Fluconazole category by CLSI M27* Defined resistance mechanism N of isolates N of isolates with DDT result for fluconazole % CA N (%) of isolates with discrepancies


FR F-SDD FS VME ME Minor
C. albicans FR ERG11, K143R or G464S variant 7 5 0 2
FS No variants (ERG11, TAC1, MRR1, and UPC2) defined 59 0 0 59
Total 66 5 0 61 97.0 2 (3.0) 0 (0.0) 0 (0.0)
C. glabrata FR PDR1 variants 33 33 0 0
F-SDD No PDR1 variants 36 1 35 0
Total 69 34 35 0 98.6 0 (0.0) 0 (0.0) 1 (1.4)
C. parapsilosis FR ERG11, Y132F variant 15 12 2 1
FS No variants (ERG11, TAC1, MRR1, and UPC2) defined 14 0 0 14
Total 29 12 2 15 89.7 1 (3.4) 0 (0.0) 2 (6.9)
C. tropicalis FR ERG11 overexpression or Y132F variant 8 7 1 0
FS No variants (ERG11 and UPC2) defined 16 0 0 16
Total 24 7 1 16 95.8 0 (0.0) 0 (0.0) 1 (4.2)
Total FR Presence of defined azole resistance mechanisms 63 57 3 3
FS/F-SDD No azole variant defined 125 1 35 89
Total 188 58 38 92 96.3 3 (1.6) 0 (0.0) 4 (2.1)

*Fluconazole susceptibility testing was performed according to the CLSI M27 BMD-ED4 method [15]. Categorical results were determined using the CLSI M60-ED2 method [16]. Fluconazole susceptibility testing was performed according to the CLSI M44-ED3 method [23]. Categorical results were determined using the CLSI M60-ED2 method [16].

Abbreviations: BMD, broth microdilution; DDT, disk diffusion testing; CA, categorical agreement; FR, fluconazole resistant; FS, fluconazole susceptible; F-SDD, fluconazole-susceptible dose-dependent; ME, major error; Minor, minor error; VME, very major error.

Ann Lab Med 2021;41:559~567 https://doi.org/10.3343/alm.2021.41.6.559

© Ann Lab Med