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Table. 2.

Table. 2.

EliA, QUANTA Flash, IIFA, and anti-ENA antibody assay results in samples with discrepant automated immunoassay and IIFA results

Case number Diagnosis EliA* QUANTA Flash IIFA Anti-ENA antibodies
1 SLE (P) 7.5 (P) > 200.0 (N) 1 : 40 SS-A (Ro60)
2 SLE (P) 7.3 (P) 125.5 (N) 1 : 40 SS-A (Ro60)
3 CTD (N) 0.4 (P) > 200.0 (N) Weak SS-A (Ro60)
4 SjS (P) 1.5 (P) 29.5 (N) 1 : 40 SS-A (Ro60)
5 SLE (N) 0.4 (N) 8.1 (P) 1 : 640 Nucleosome
6 SLE (N) 0.5 (N) 9.8 (P) 1 : 320 Nucleosome
7 SLE (P) 8.0 (P) 49.1 (N) Weak Histone, SS-A (Ro60)
8 SLE (P) 5.7 (P) 111.4 (N) 1 : 40 Ribosomal-P, SS-A (Ro60)
9 SLE (P) 4.7 (N) 17.7 (P) 1 : 160 dsDNA, nRNP, ribosomal-P, Sm
10 SLE (N) 0.9 (N) 8.7 (P) 1 : 320 Histone
11 SLE (N) 0.7 (N) 11.0 (P) 1 : 80 Scl-70
12 SLE (N) 0.6 (N) 11.7 (P) 1 : 1,280 ND
13 SLE (N) 0.4 (N) 10.8 (P) 1 : 320 ND
14 SLE (N) 0.3 (N) 15.4 (P) 1 : 80 ND
15 SLE (N) 0.1 (N) 10.3 (P) 1 : 80 ND
16 SLE, SjS (N) 0.1 (N) 8.8 (P) 1 : 80 ND
17 CTD (N) 0.4 (N) 2.6 (P) 1 : 320 ND
18 CTD (N) 0.2 (N) 5.7 (P) 1 : 80 ND
19 Unclassified CTD (N) 0.2 (N) 8.4 (P) 1 : 160 ND
20 Collagen disease (N) 0.1 (N) 3.9 (P) 1 : 320 ND
21 RA (N) 0.1 (N) 8.1 (P) > 1 : 1,280 ND
22 RA (N) 0.1 (N) 7.4 (P) 1 : 160 ND
23 SjS (N) 0.4 (N) 4.1 (P) 1 : 80 ND
24 SSc (N) 0.3 (N) 5.5 (P) 1 : 320 ND
25 Arthralgia (N) 0.3 (N) 6.6 (P) 1 : 80 ND
26 Arthrosis (N) 0.2 (N) 3.8 (P) 1 : 80 ND
27 Cough (N) 0.1 (N) 4.5 (P) 1 : 160 ND
28 Dry mouth (N) 0.2 (N) 7.5 (P) 1 : 160 ND
29 Hemoptysis (N) 0.2 (N) 6.9 (P) 1 : 80 ND
30 ILD (N) 0.2 (N) 3.8 (P) 1 : 80 ND
31 Oral ulcer (N) 0.2 (N) 3.0 (P) 1 : 160 ND
32 R/O FFA (N) 0.2 (N) 4.2 (P) 1 : 160 ND
33 SLE (N) 0.2 (P) 24.1 (N) Weak ND
34 SLE (N) 0.9 (P) 24.1 (N) 1 : 40 ND
35 Arthrosis (N) 0.1 (P) 44.7 (N) 1 : 40 ND
36 Rash (N) 0.2 (P) 131.7 (N) Negative ND
37 CTD (P) 1.1 (N) 13.6 (N) Weak ND
38 OA (P) 1.7 (N) 7.8 (N) 1 : 40 ND
39 SLE (P) 1.7 (N) 12.7 (N) 1 : 40 ND
40 RA (P) 1.1 (P) 24.6 (N) 1 : 40 ND
41 Thrombocytopenia (P) 1.2 (P) 51.2 (N) 1 : 40 ND
42 CTD (P) 1.1 (N) 6.8 (P) 1 : 80 ND

*EliA: >1.0 ratio, positive; 0.7–1.0 ratio, equivocal; <0.7 ratio, negative. QUANTA Flash: ≥20.0 CU, positive; <20.0 CU, negative. IIFA: ≥1:80, positive; <1:80, negative.

Abbreviations: CTD, connective tissue disease; CU, chemiluminescent units; ENA, extractable nuclear antigen; FFA, Frontal fibrosing alopecia; IIFA, indirect immunofluorescence assay; ILD, interstitial lung disease; N, negative; ND, not detected; OA, osteoarthritis; P, positive; RA, rheumatoid arthritis; SLE, systemic lupus erythematosus; SjS, Sjögren’s syndrome; SSc, systemic sclerosis; R/O, rule out; SjS, primary Sjögren’s syndrome; SLE, systemic lupus erythematosus; SSc, systemic sclerosis.

Ann Lab Med 2022;42:63~70

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