Table. 3. Table 3

QC values and AMR for selected LC-MS/MS assays reported in the literature

Assay	Analyte	QC1	QC2	QC3	QC4	LLOQ	ULOQ	Unit	Reference
Thyroglobulin in serum	Thyroglobulin	3.8	5.5	22.3	NA	2	20	ng/mL	[111]
		7.1	23.7	57.4	NA	0.8	100		[112]
		2.3	6.5	172.8	NA	0.5	1,045		[113]
		0.1	0.5	4	NA	0.02	20		[114]
Testosterone in serum	Testosterone	0.44	12.11	37.34	0.86*	0.035	69.34	nM	[115]
Testosterone in serum	Testosterone	0.415	7.23	NA	NA	0.0346	138.4	nM	[116]
E1 and E2 in serum	Estrone (E1)	3	30	NA	NA	1.2	156.8	pg/mL	[117]
	Estrone (E1)	7.82	16.71	77.17	208.93	5	200	pg/mL	[118]
	Estradiol (E2)	1.5	22.5	NA	NA	1.3	56.5	pg/mL	[117]
	Estradiol (E2)	8.37	17.92	77.28	205.8	5	200	pg/mL	[118]
25-OH Vitamin D in serum	25-OH Vitamin D3	27	118	209	NA	25	545	nM	[50]
		55	87	NA	NA	5	316		[119]
		31	62	125	NA	1.2	211		[120]
	25-OH Vitamin D2	36	120.5	205	NA	25	545	nM	[50]
		52	76	NA	NA	4	250		[119]
		30	61	121	NA	1.2	205		[120]
Immunosuppressants in whole blood	Tacrolimus	1.9	46.7	97.3	NA	0.5	150	ng/mL	[121]
		2.9	7.6	16	34	0.6	44.7		[122]
		5	15	40	NA	1	50		[123]
	Sirolimus	1.9	24	76.9	NA	0.5	100	ng/mL	[12]
		2.7	10	20	40	0.6	51.4		[122]
		5	15	40	NA	2.5	50		[123]
	Cyclosporine A	50	250	480	1,120	11.6	1,850	ng/mL	[122]
	Cyclosporine A	50	800	1,600	NA	10	2,000	ng/mL	[123]
	Everolimus	2.6	5	10	34	0.6	46.3	ng/mL	[122]
	Everolimus	5	15	40	NA	2.5	50	ng/mL	[123]

*QC4 is used only when assaying samples from females.

Abbreviations: LC-MS/MS, liquid chromatography-tandem mass spectrometry; QC, quality control; LLOQ, lower limit of quantification; ULOQ, upper limit of quantification; AMR, analytical measurement range; NA, not applicable.

Ann Lab Med 2022;42:121~140 https://doi.org/10.3343/alm.2022.42.2.121