pISSN 2234-3806eISSN 2234-3814
Table. 1.
Summary of column dimensions, system maximum pressures, temperature and maximum flow rate for multiple examples of LC-MS/MS assays utilized in clinical laboratories globally
| Reference | [43] | [44] | [45] | [46] | [47] | [48] | [49] | [50] | [51] | [52] | [53] |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Length (mm) | 100 | 50 | 150 | 100 | 125 | 100 | 35 | 50 | 100 | 50 | 100 |
| Internal diameter (mm) | 2.1 | 2 | 2.1 | 2 | 4 | 2 | 0.5 | 2.1 | 2.1 | 4.6 | 3 |
| Particle diameter (μm) | 5 | 3 | 1.7 | 3 | 5 | 3 | 3.5 | 1.7 | 3.5 | 2.6 | 2.6 |
| System pressure max (bar) | 400 | 250 | 600 | 1,000 | 350 | 600 | 600 | 1,000 | 400 | 600 | 1,000 |
| Guard column used? | Yes | Yes | ND | ND | ND | ND | Yes | ND | ND | ND | ND |
| Temp (°C) | 40 | ND | ND | ND | 30 | 30 | 40 | 45 | 30 | ND | 45 |
| Flow rate max (μL/min) | 400 | 400 | 500 | 300 | 850 | 600 | 40 | 350 | 800 | ND | ND |
*Note the consistency in which multiple practitioners apply internal diameters ≥2 mm, perhaps to address particle/stationary phase heterogeneity.
Abbreviation: ND, not described in the referenced study.
Ann Lab Med 2022;42:121~140 https://doi.org/10.3343/alm.2022.42.2.121
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