pISSN 2234-3806eISSN 2234-3814
Table. 2.
Summary of the questionnaire on the current practice on the pre-analytical phase of plasma
| Object | Questions | Choices | N (%) of laboratories |
|---|---|---|---|
| 1. All laboratories (N=19) | 1.1. What blood collection tube do you use? | a. K2 EDTA tube | 9 (47.4) |
| b. K3 EDTA tube | 1 (5.3) | ||
| c. Tubes with cell stabilizer | 8 (42.1) | ||
| d. No response | 1 (5.3) | ||
| 1.2. How much whole blood is collected? | a. 5 mL | 3 (15.8) | |
| b. 5 or 10 mL | 1 (5.3) | ||
| c. 6.5 – 8.5 mL | 3 (15.8) | ||
| d. 9 mL | 2 (10.5) | ||
| e. 10 mL | 6 (31.6) | ||
| f. 12 mL | 3 (15.8) | ||
| g. No response | 1 (5.3) | ||
| 2. K2 or K3 EDTA tube user (N=10) |
2.1. What is the time interval between blood collection and plasma separation? | a. Within 2 hr | 6 (60.0) |
| b. Within 4 hr | 4 (40.0) | ||
| 2.2. How many centrifugation steps do you use to separate the plasma? | a. One | 4 (40.0) | |
| b. Two | 6 (60.0) | ||
| 2.3. 1st centrifugation method (force and time) | a. ≥1,000 – <2,000 ×g for 10 min | 2 (20.0) | |
| b. ≥2,000 – <3,000 ×g for 5 min | 2 (20.0) | ||
| c. ≥2,000 – <3,000 ×g for 10 min | 1 (10.0) | ||
| d. ≥3,000 – <4,000 ×g for 5 min | 2 (20.0) | ||
| e. ≥3,000 – <4,000 ×g for 10 min | 1 (10.0) | ||
| f. No response | 2 (20.0) | ||
| 2.4. 2nd centrifugation method (force and time) | a. ≥2,000 – <3,000 ×g for 10 min | 1 (10.0) | |
| b. ≥3,000 – <4,000 ×g for 10 min | 2 (20.0) | ||
| c. ≥10,000 ×g for 5 min | 1 (10.0) | ||
| d. ≥10,000 ×g for 10 min | 1 (10.0) | ||
| e. Not done | 4 (40.0) | ||
| f. No response | 1 (10.0) | ||
| 2.5. On average, how long does it take to perform a test after plasma separation? | a. Within a day | 1 (10.0) | |
| b. Within two days | 3 (30.0) | ||
| c. Within three days | 1 (10.0) | ||
| d. More than three days | 5 (50.0) | ||
| 2.6. At what temperature do you store separated plasma samples if the test cannot be performed immediately? | a. 2~8°C | 1 (10.0) | |
| b. −20°C | 2 (20.0) | ||
| c. −70 ~ −80°C | 5 (50.0) | ||
| d. No response | 2 (20.0) | ||
| 3. Users of tubes with cell stabilizer (N=8) | 3.1. Do you perform inversions to mix the sample with anticoagulant and stabilizer solutions after blood collection? | a. Yes | 8 (100.0) |
| b. No | 0 | ||
| 3.2. Do you know the instructions for storage and plasma processing of your cell stabilizing tubes? | a. Yes | 6 (75.0) | |
| b. No | 2 (25.0) |
Abbreviation: EGFR, epidermal growth factor receptor.
Ann Lab Med 2022;42:141~149 https://doi.org/10.3343/alm.2022.42.2.141
© Ann Lab Med