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Table. 2.

Table. 2.

Summary of the questionnaire on the current practice on the pre-analytical phase of plasma EGFR variant testing in 19 Korean clinical laboratories

Object Questions Choices N (%) of laboratories
1. All laboratories (N=19) 1.1. What blood collection tube do you use? a. K2 EDTA tube 9 (47.4)
b. K3 EDTA tube 1 (5.3)
c. Tubes with cell stabilizer 8 (42.1)
d. No response 1 (5.3)
1.2. How much whole blood is collected? a. 5 mL 3 (15.8)
b. 5 or 10 mL 1 (5.3)
c. 6.5 – 8.5 mL 3 (15.8)
d. 9 mL 2 (10.5)
e. 10 mL 6 (31.6)
f. 12 mL 3 (15.8)
g. No response 1 (5.3)
2. K2 or K3 EDTA tube user
(N=10)
2.1. What is the time interval between blood collection and plasma separation? a. Within 2 hr 6 (60.0)
b. Within 4 hr 4 (40.0)
2.2. How many centrifugation steps do you use to separate the plasma? a. One 4 (40.0)
b. Two 6 (60.0)
2.3. 1st centrifugation method (force and time) a. ≥1,000 – <2,000 ×g for 10 min 2 (20.0)
b. ≥2,000 – <3,000 ×g for 5 min 2 (20.0)
c. ≥2,000 – <3,000 ×g for 10 min 1 (10.0)
d. ≥3,000 – <4,000 ×g for 5 min 2 (20.0)
e. ≥3,000 – <4,000 ×g for 10 min 1 (10.0)
f. No response 2 (20.0)
2.4. 2nd centrifugation method (force and time) a. ≥2,000 – <3,000 ×g for 10 min 1 (10.0)
b. ≥3,000 – <4,000 ×g for 10 min 2 (20.0)
c. ≥10,000 ×g for 5 min 1 (10.0)
d. ≥10,000 ×g for 10 min 1 (10.0)
e. Not done 4 (40.0)
f. No response 1 (10.0)
2.5. On average, how long does it take to perform a test after plasma separation? a. Within a day 1 (10.0)
b. Within two days 3 (30.0)
c. Within three days 1 (10.0)
d. More than three days 5 (50.0)
2.6. At what temperature do you store separated plasma samples if the test cannot be performed immediately? a. 2~8°C 1 (10.0)
b. −20°C 2 (20.0)
c. −70 ~ −80°C 5 (50.0)
d. No response 2 (20.0)
3. Users of tubes with cell stabilizer (N=8) 3.1. Do you perform inversions to mix the sample with anticoagulant and stabilizer solutions after blood collection? a. Yes 8 (100.0)
b. No 0
3.2. Do you know the instructions for storage and plasma processing of your cell stabilizing tubes? a. Yes 6 (75.0)
b. No 2 (25.0)

Abbreviation: EGFR, epidermal growth factor receptor.

Ann Lab Med 2022;42:141~149 https://doi.org/10.3343/alm.2022.42.2.141

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