pISSN 2234-3806eISSN 2234-3814
Table. 3.
Comparison of turnaround time between DI-60 and manual counting for WBC differentials
| Process step | TAT (min:sec) (median, IQR) | |||||
|---|---|---|---|---|---|---|
| Total (N=40) | Normal (N=10) | Mild leukopenia (N=10) | Moderate leukopenia (N=10) | Severe leukopenia (N=10) | ||
| DI-60 analysis | 1. Insert a slide in the instrument | 0:33 (0:32-0:34) | 0:33 (0:33-0:34) | 0:33 (0:32-0:33) | 0:33 (0:32-0:33) | 0:33 (0:33-0:34) |
| 2. Scan ideal zone under low power (10 ×) | 0:18 (0:17-0:19) | 0:18 (0:17-0:19) | 0:18 (0:18-0:20) | 0:18 (0:17-0:18) | 0:19 (0:17-0:19) | |
| 3. Acquire images under high power (100 ×) (pre-classification) | 2:09 (1:58-2:21) | 2:05 (1:58-2:19) | 2:27 (2:08-2:43) | 2:18 (2:08-2:25) | 1:48 (1:37-1:57) | |
| 4. Verification | 1:53 (0:45-2:45) | 2:03 (1:45-2:27) | 3:09 (2:15-4:02) | 2:11 (1:21-3:00) | 0:20 (0:15-0:27) | |
| Total |
5:00 (2:36-6:06) | 5:05 (4:38-5:23) | 6:29 (5:36-7:28) | 5:13 (4:35-6:36) | 3:02 (2:46-3:14) | |
| Manual counting |
1. Place a slide on the microscope stage | 0:03 (0:03-0:04) | 0:03 (0:03-0:04) | 0:03 (0:03-0:04) | 0:04 (0:03-0:04) | 0:03 (0:03-0:04) |
| 2. Scan ideal zone under low power (100 ×) | 0:09 (0:05-0:10) | 0:05 (0:04-0:09) | 0:07 (0:05-0:08) | 0:09 (0:06-0:10) | 0:12 (0:10-0:13) | |
| 3. Count cells under high power (1,000 ×) | 2:42 (1:54-3:52) | 1:47 (1:21-2:01) | 2:36 (2:01-3:30) | 3:02 (2:32-3:59) | 4:30 (3:32-5:21) | |
| 4. Record results | 0:18 (0:14-0:20) | 0:17 (0:15-0:20) | 0:19 (0:17-0:23) | 0:18 (0:14-0:21) | 0:11 (0:09-0:21) | |
| Total |
3:11 (2:18-4:21) | 2:11 (1:46-2:35) | 3:02 (2:30-4:04) | 3:27 (3:03-4:28) | 5:00 (4:01-5:46) | |
The last process step of data transfer to the laboratory information system was excluded for both DI-60 analysis and manual counting.
*Total value indicates median and IQR of total TAT for counting total cells in each slide in total group and each group; †We only compared TAT for one analytical process of one expert in manual counting; if the result of manual counting would have been determined as the mean of the results obtained by the two experts, the TAT of manual counting would have been significantly longer.
Abbreviations: WBC, white blood cell; TAT, turnaround time; IQR, interquartile range; N, number.
Ann Lab Med 2022;42:398~405 https://doi.org/10.3343/alm.2022.42.4.398
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