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Table. 6.

Table. 6.

Example analyte-to-IS ratio of a sample diluted by under-aliquoting of the sample as opposed to volumetric dilution of the sample by addition of solvent

Sample volume (µL) Analyte concentration, sample (nM) IS concentration (nM) IS volume (µL) Final volume (µL) Analyte concentration, final mixture (nM) IS concentration, final mixture (nM) Analyte-to-IS ratio
100 5,000 100 500 600 833.33 83.33 10
10 5,000 100 500 510 98.04 98.04 1

Note that the analyte-to-IS ratio is preserved despite reducing the volume of the sample aliquot.

Abbreviation: IS, internal standard.

Ann Lab Med 2022;42:531~557 https://doi.org/10.3343/alm.2022.42.5.531

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