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Table. 9.

Table. 9.

Chromatographic program for the original validated gradient for 11-nor-9-carboxy-Δ9-tetrahydrocannabinol and the modified gradient to provide resolution of the delta-8 isomer generated as a reflex test for samples with inadequate resolution

Originally validated gradient Modified reflex gradient


Time (min) Flow rate (mL/min) %A %B Time (min) Flow rate (mL/min) %A %B
0 0.5 100 0 0 0.55 100 0
0.05 0.5 41 59 0.05 0.55 48 52
1 0.5 41 59 1.45 0.55 48 52
1.9 0.5 38 62 3 0.55 45 55
1.95 0.5 0 100 3.15 0.55 0 100
2.15 0.7 0 100 3.3 0.7 0 100
2.23 0.7 100 0 3.5 0.7 0 100
2.3 0.7 100 0 3.55 0.7 100 0
3.65 0.7 100 0
Ann Lab Med 2022;42:531~557 https://doi.org/10.3343/alm.2022.42.5.531

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