eISSN 2234-3814

Table. 1.

Table. 1.

Example features to be evaluated prior to sample extraction or LC-MS/MS analysis

Component Required information
Assay reagent Calibration expiry Expiration date
QC expiry Expiration date
Critical reagent expiry Expiration date
Critical reagent sufficient volume Number of samples and volume used per sample
Sample Sample volume Sufficient volume and insufficient quantity SOP
Sample temperature Outside of SOP requirements (e.g., sample received at room temperature instead of frozen)
Sample interferences Gross hemolysis, lipemia, icteria, etc.
Sample stability Date of draw and stability SOP
Sample tube type Colored cap or subaliquot identifier for type
Sample pertinent information History of sample, if provided. Monitoring of certain patients may indicate gross elevations in the compound(s). Predilution may prevent the need to address carryover or subsequent re-assay on dilution
Sample previous analysis Freeze–thaw cycles and pertinent prior results (e.g., dilution required)
Sample abnormal observations Bacterial contamination, inappropriate color, etc.
System Mobile phase(s) expiry Expiration date
Mobile phase volume Sufficient volume
Mobile phase abnormal observations Odd material in bottle (dust, bacteria), filter stones above liquid level
Column check Column manufacturer, dimensions, and stationary phase
MS gas pressures/volume Pressure within range and sufficient volume of gas available
MS base pressure System pressure within range
Autosampler wash expiry Expiration date
Autosampler wash volume Sufficient volume
System suitability review See Table 2

Abbreviations: LC-MS/MS, liquid chromatography-tandem mass spectrometry; QC, quality control; SOP, standard operating procedure.

Ann Lab Med 2022;42:531~557

© Ann Lab Med