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Table. 2.

Table. 2.

Description of available data components derived from triplicate analysis of SST injections

Data component Acute determination Longitudinal determination Possible trouble shooting target(s)
IS Peak width above baseline Gross deviation Increasing trend Column, LC pumps, tubing, mobile phases
Peak width at 50% height Gross deviation Increasing trend Column, LC pumps, tubing, mobile phases
Peak height Gross deviation Increasing trend Autosampler, MS source, MS optics
Peak area reproducibility Poor precision Increasing trend Autosampler, MS source
Retention time Gross deviation Increasing or decreasing trend Column, LC pumps, tubing, SST solvent
Analyte Peak width above baseline Gross deviation Increasing trend Column, LC pumps, tubing, mobile phases
Peak width at 50% height Gross deviation Increasing trend Column, LC pumps, tubing, mobile phases
Peak height Gross deviation Increasing trend Autosampler, MS, mobile phases
Retention time Gross deviation Increasing or decreasing trend LC pumps, tubing, SST solvent
Peak area Confidence in appropriate data analysis for batch (enough response) MS source, MS optics, mobile phases, autosampler
LC system Pressure trace Min/max outside of normal operating range Initial (equilibrated conditions) increasing or decreasing trend Column, LC, tubing
Other Known interferences with resolution calculated Minimum resolution achieved Change in resolution over time Column, mobile phases, LC

Abbreviations: LC, liquid chromatography; MS, mass spectrometry; SST, system suitability test; IS, internal standard.

Ann Lab Med 2022;42:531~557 https://doi.org/10.3343/alm.2022.42.5.531

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