Fig. 2. NK cells cultured in different culture media exhibit different receptor expression levels. (A) Histogram of cytotoxic effector molecule and surface receptor expression of UCB NK cells cultured RC (orange), DS (blue), or AI (red). The geometric means are indicated in the respective histograms. (B) Percent expression levels of cytotoxic effector molecules and surface receptors on day 28 (N=9). (C) Geometric mean expression levels of cytotoxic effector molecules and surface receptors on day 28 (N=9) (*P<0.05; **P<0.01; ***P<0.001).
Abbreviations: RC, RPMI medium+FBS; DS, DMEM+Ham’s F12+various supplements; AI, AIM V+ICSR; UCB, umbilical cord blood; NK, natural killer; RPMI, Roswell Park Memorial Institute; FBS, fetal bovine serum; DMEM, Dulbecco’s modified Eagle’s medium, ICSR, Immune Cell Serum Replacement.

Ann Lab Med 2022;42:638~649 https://doi.org/10.3343/alm.2022.42.6.638