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Fig. 3.

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Fig. 3. Culture medium shifting is advantageous for restoring NK cell function. (A) Fold expansion of UCB NK cells cultured in DS (blue), DS-AI (purple), AI-DS (green), or AI (red) (N=8) on day 28. (B) Purity (CD56+/CD3− NK cells) of media-shifted UCB-expanded NK cells cultured in DS (blue), DS-AI (purple), AI-DS (green), or AI (red) (N=11) on day 28. Flow cytometry-based cytotoxicity assay of UCB-expanded NK cells cultured in DS, AI, AI-DS, or DS-AI at the indicated ratios; (C) K562 cells, N=7; (D) Raji cells, N=4; (E) Raji cells + rituximab, N=7 (C–E). *, †P<0.05; **, ††P<0.01; ***, †††P<0.001; *DS vs. AI; DS vs. DS-AI; AI vs. AI-DS.
Abbreviations: RC, RPMI medium+FBS; DS, DMEM+Ham’s F12+various supplements; AI, AIM V+ICSR; DS-AI, DS shifted to AI on day 14; AI-DS, AI shifted to DS on day 14; UCB, umbilical cord blood; NK, natural killer; RPMI, Roswell Park Memorial Institute; FBS, fetal bovine serum; DMEM, Dulbecco’s modified Eagle’s medium, ICSR, Immune Cell Serum Replacement.
Ann Lab Med 2022;42:638~649 https://doi.org/10.3343/alm.2022.42.6.638

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