Assigned Ct values and uncertainties of the synthetic norovirus GI and GII RNAs using the AccuPower Norovirus Real-Time RT-PCR Kit
Storage temperature | –70°C | –20°C | ||
---|---|---|---|---|
Material | Norovirus GI | Norovirus GII | Norovirus GI | Norovirus GI |
Mean ± SD |
20.74 ± 0.48 | 19.04 ± 0.71 | 20.74 ± 0.48 | 19.04 ± 0.71 |
Total replicates | 398 | 398 | 398 | 398 |
ubb | 0.55 | 0.37 | 0.55 | 0.37 |
ustab | 0.18 | 0.21 | 0.21 | 0.22 |
uchar | 0.09 | 0.14 | 0.09 | 0.14 |
uCRM | 0.59 | 0.45 | 0.60 | 0.45 |
Degrees of freedom | 9 | 14 | 9 | 15 |
k | 2.26 | 2.00 | 2.26 | 2.00 |
U | 1.33 | 0.89 | 1.35 | 0.90 |
*Mean is the average value of the characteristic values and was constant regardless of the measured value according to the temperature.
Abbreviations: SD, standard deviation; k, coverage factor; U, combined uncertainty; ubb, standard uncertainty associated with homogeneity; ustab, standard uncertainty associated with a value from a stability study; uchar, standard uncertainty associated with a value assigned in a characterization study; uCRM, uncertainty associated with a property value of the certified reference material.
© Ann Lab Med