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Table. 3.

Table. 3.

Assigned Ct values and uncertainties of the synthetic norovirus GI and GII RNAs using the AccuPower Norovirus Real-Time RT-PCR Kit

Storage temperature –70°C –20°C



Material Norovirus GI Norovirus GII Norovirus GI Norovirus GI
Mean ± SD* 20.74 ± 0.48 19.04 ± 0.71 20.74 ± 0.48 19.04 ± 0.71
Total replicates 398 398 398 398
ubb 0.55 0.37 0.55 0.37
ustab 0.18 0.21 0.21 0.22
uchar 0.09 0.14 0.09 0.14
uCRM 0.59 0.45 0.60 0.45
Degrees of freedom 9 14 9 15
k 2.26 2.00 2.26 2.00
U 1.33 0.89 1.35 0.90

*Mean is the average value of the characteristic values and was constant regardless of the measured value according to the temperature.

Abbreviations: SD, standard deviation; k, coverage factor; U, combined uncertainty; ubb, standard uncertainty associated with homogeneity; ustab, standard uncertainty associated with a value from a stability study; uchar, standard uncertainty associated with a value assigned in a characterization study; uCRM, uncertainty associated with a property value of the certified reference material.

Ann Lab Med 2023;43:38~44

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