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Table. 1. Table 1

Result of identification and antifungal susceptibility testing of two Candida isolates from a pediatric patient with congenital megacolon

Test method	Isolate 1	Isolate 2
Identification
Vitek2 YST card (bioMérieux, Marcy-l’Étoile, France)	Candida duobushaemulonii	C. duobushaemulonii
MALDI-TOF VITEK MS (bioMérieux)	C. duobushaemulonii	C. duobushaemulonii
MALDI-TOF MS, Biotyper (Biotyper; Bruker Daltonics, Billerica, MA, USA)	Candida pseudohaemulonii	C. pseudohaemulonii
MALDI-TOF MS, ASTA MicroIDSys (ASTA, Suwon, Korea)	C. pseudohaemulonii	C. pseudohaemulonii
ITS sequence analysis (identical rate to reference GenBank No.)	Candida vulturna (100% [438/438 bp] identity to MN330068.1)	C. vulturna (100% [438/438 bp] identity to MN330068.1)
	C. pseudohaemulonii (95% [417/441 bp] identity to NR_163771.1)	C. pseudohaemulonii (95% [417/441 bp] identity to NR_16 3771.1)
D1/D2 domain sequence analysis (identical rate to reference GenBank No.)	C. vulturna (100% [482/482 bp] identity to MN197921.1)	C. vulturna (100% [482/482 bp] identity to MN197921.1)
	C. pseudohaemulonii (96% [464/484 bp] identity to EF177490.1)	C. pseudohaemulonii (96% [464/484 bp] identity to EF177490.1)
Antifungal susceptibility (MIC, μg/mL)
Amphotericin B (Etest)	8	12
Fluconazole (CLSI M27-ED4)	4	4
Voriconazole (CLSI M27-ED4)	0.25	0.25
Micafungin (CLSI M27-ED4)	0.5	0.5
Caspofungin (CLSI M27-ED4)	0.5	0.5

Abbreviations: MALDI-TOF MS, matrix-associated laser desorption/ionization time-of-flight mass spectrometry; ITS, internal transcribed spacer region; D1/D2 domain, D1/D2 regions of the 26S rRNA gene; MIC, minimum inhibitory concentration; CLSI M27-ED4, CLSI M27-ED4 broth microdilution method.

Ann Lab Med 2023;43:395~397 https://doi.org/10.3343/alm.2023.43.4.395