pISSN 2234-3806eISSN 2234-3814
Table. 2.
Comparison of real-time PCR Ct values for respiratory viruses and human RNase P
| Virus | NPS (Noble Bio) in transport medium | NPS (Copan) in transport medium | Nasal swab in transport medium (five swab rubs) | Saliva swab in transport medium | Saliva (no transport medium) | |
|---|---|---|---|---|---|---|
| SARS-CoV-2 (N = 34) | ||||||
| 23.3 (21.5–27.7) | 24.5 (21.6–31.1) | 29.1 (25.9–34.1) | 35.4 (29.6–40.0) | 34.6 (28.3–36.6) | < 0.00001 | |
| 25.8 (24.3–30.7) | 27.6 (23.7–32.5) | 32.1 (28.4–36.9) | 37.4 (32.7–40.0) | 33.5 (30.2–38.6) | < 0.00001 | |
| 23.5 (22.1–28.4) | 25.4 (22.0–31.2) | 30.7 (27.2–34.2) | 36.0 (30.5–40.0) | 31.0 (28.5–37.2) | < 0.00001 | |
| HRV (N = 5) | 24.7 (24.0–32.6) | 25.1 (24.2–30.1) | 27.8 (25.4–33.5) | 38.0 (36.4–40.2) | NA | 0.00073 |
| HEV (N = 2) | 33.5 (33.4–33.6) | 37.1 (24.2–40.0) | 34.6 (30.3–38.8) | 38.5 (38.0–39.0) | NA | 0.142 |
| PIV3 (N = 2) | 31.1 (26.2–36.1) | 33.6 (29.3–38.0) | 36.1 (32.3–40.0) | 37.2 (34.3–40.0) | NA | 0.0079 |
| PIV4 (N = 1) | 28.9 | 30.7 | 29.8 | 34.0 | NA | |
| RSV B (N = 1) | 28.7 | 25.3 | 29.6 | 38.9 | NA | |
| Adenovirus (N = 1) | 22.3 | 28.2 | 29.7 | 40.0 | NA | |
| HBoV (N = 1) | 22.41 | 13.7 | 27.49 | 36.9 | NA | |
| Influenza A virus (N = 1) | 36.1 | 32.9 | 37.7 | 40.0 | NA | |
| No virus (N = 8) | NA | NA | NA | NA | NA | |
| RNase P (N = 56) | 32.9 (32.1–34.7) | 33.3 (31.8–34.7) | 34.6 (32.7–36.3) | 32.7 (31.0–34.2) | 28.7 (27.6–31.2) | < 0.00001 |
The bold text indicates the lowest median Ct value (highest viral load) of real-time PCR among the five collection methods. Tests with negative results were defined as Ct=40. Data are expressed as medians (first to third quartiles).
Abbreviations: Ct, cycle threshold of real-time PCR; HBoV, human bocavirus; HEV, human enterovirus; HRV, human rhinovirus; NA, not applicable; NPS, nasopharyngeal swab; PIV3, parainfluenza virus type 3; PIV4, parainfluenza virus type 4; RSV, respiratory syncytial virus; SARS-CoV-2, severe acute respiratory syndrome-coronavirus 2; RdRP, RNA-dependent RNA polymerase.
Ann Lab Med 2023;43:434~442 https://doi.org/10.3343/alm.2023.43.5.434
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