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Table. 3.

Table. 3.

Changes in the assay performance based on optimized co values*

Assay Original co Optimized co Sensitivity, % Specificity, %
Negative Positive Original co (CI) Optimized co (CI) Original co (CI) Optimized co (CI)
CLIA VirC <0.9 >1.1 Lowered to ≥0.3 78 (67–89) 86 (76–96) 100 (100–100) 100 (100–100)
ELISA DRG <9 >11 Lowered to ≥7 72 (60–84) 80 (69–91) 100 (100–100) 96 (91–100)
ELISA Euro <0.8 ≥1.1 Lowered to ≥0.8 80 (69–91) 90 (82–98) 98 (94–100) 98 (94–100)
ELISA IBL <9 >11 Raised to ≥15 88 (79–97) 84 (74–94) 80 (69–91) 94 (87–100)
ELISA rBio <0.9 >1.1 Lowered to ≥0.5 64 (51–77) 86 (76–96) 100 (100–100) 98 (94–100)
ELISA VirC <9 >11 Lowered to ≥3 68 (55–81) 90 (82–98) 100 (100–100) 98 (94–100)

*The original co values represent the manufacturers’ recommendations, and the optimized co values relied on the YI calculations. When two co values had the same YI, the co with higher specificity was selected.

Abbreviations: co, cut-off; CI, 95% confidence interval; CLIA, chemiluminescence immunoassay; YI, Youden’s index.

Ann Lab Med 2024;44:155~163 https://doi.org/10.3343/alm.2023.0212

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