Table. 1.

Table 1

Detection of a high-dose hook effect using 1:10, 1:50, and 1:400 dilutions

Sample number	Neat (nmol/L)	1:10 dilution (nmol/L)	Hook effect detected (1:10; >168.54 nmol/L)	1:50 dilution (nmol/L)	Hook effect detected (1:50; >168.54 nmol/L)	1:400 dilution (nmol/L)
1	48.89	125.79	No hook effect	154.83	No hook effect
2	41.63	1,049.21	Detected	2,412.81	Detected
3	10.15	274.04	Detected	2,901.46	Detected
4	85.34	74.78	No hook effect	85.45	No hook effect
5	55.85	197.98	Detected	247.36	Detected
6	64.27	>1,685.40	Detected	2,429.38	Detected
7	42.74	>1,685.40	Detected	>8,426.97	Detected	21,166.57
8	25.99	80.62	No hook effect	115.79	No hook effect
9	110.28	1,140.84	Detected	1,316.69	Detected
10	43.51	>1,685.40	Detected	6,093.54	Detected
11	20.08	732.08	Detected	5,770.34	Detected
12	89.61	111.97	No hook effect	110.79	No hook effect
13	20.31	282.58	Detected	530.56	Detected
14	30.52	>1,685.40	Detected	>8,426.97	Detected	31,265.56
15	34.17	459.78	Detected	824.10	Detected
16	28.51	>1,685.40	Detected	>8,426.97	Detected	12,656.46
17	34.17	>1,685.40	Detected	>8,426.97	Detected	15,379.49
18	9.56	901.91	Detected	>8,426.97	Detected	21,466.80
19	28.60	1,326.57	Detected	6,596.07	Detected
20	>168.54	>1,685.40	Detected	3,221.52	Detected
21	31.82	1,133.48	Detected	5,771.01	Detected
22	48.18	952.13	Detected	2,553.48	Detected
23	16.34	384.89	Detected	3,048.03	Detected
24	61.57	1,084.78	Detected	1,499.72	Detected
25	8.31	302.87	Detected	2,155.17	Detected
26	28.19	413.99	Detected	613.09	Detected
27	>168.54	>1,685.40	Detected	2,070.11	Detected
28	7.66	265.34	Detected	390.22	Detected
29	10.99	75.96	No hook effect	152.87	No hook effect
30	63.43	>1,685.40	Detected	6,177.75	Detected
31	96.24	395.45	Detected	431.01	Detected
32	31.87	552.81	Detected	1,724.72	Detected
33	77.53	1,501.52	Detected	2,090.51	Detected
34	108.71	224.44	Detected	259.21	Detected
35	>168.54	1,316.69	Detected	1,617.02	Detected

Ann Lab Med 2024;44:367~370 https://doi.org/10.3343/alm.2023.0427