Fig. 1. B-cell flow cytometry crossmatch testing results. Sera from three groups, RTX+/DSA– (A), RTX+/DSA+ (B), and RTX−/DSA+ (C), were crossmatched with lymphocytes treated with the indicated pronase concentrations. The gray circles represent the results for each sample, and the black lines represent the median and interquartile range. The dotted line indicates the median channel shift (MCS) cutoff (120) for positive results. The median MCSs of the 2, 3, and 4 mg/mL pronase groups were compared with that of the 1 mg/mL pronase group using the Kruskal–Wallis test, followed by Dunn’s multiple-comparison test. The table below each graph shows the total number of samples tested (Total), the number of positive samples (Pos.), and the percentage of positive results (Pos. %). *P<0.05; ****P<0.0001.
Abbreviations: RTX, rituximab; DSA, donor HLA-specific antibody; ns, not significant.

Ann Lab Med 2024;44:545~552 https://doi.org/10.3343/alm.2024.0132