Fig. 3. T-cell flow cytometry crossmatch testing results. Sera from the RTX+/DSA– (A), RTX+/DSA+ (B and D), and RTX−/DSA+ (C and E) groups were crossmatched with lymphocytes treated with the indicated pronase concentrations. The crossmatching results for RTX+/DSA+ and RTX−/DSA+ sera were plotted in separate graphs based on the MHC class of the DSAs present in each serum sample because MHC class II molecules are usually not expressed on naive T cells (B and C for MHC class I; D and E for MHC class II). The dotted lines indicate the median channel shift (MCS) cutoff (90) for positive results. The median MCSs of each column were compared with that of the control column (0 mg/mL pronase) using the Kruskal–Wallis test, followed by Dunn’s multiple-comparison test. The table below each graph shows the total number of samples tested (Total), the number of positive samples (Pos.), and the percentage of positive results (Pos. %). *P<0.05; **P<0.01.
Abbreviations: RTX, rituximab; DSA, donor HLA-specific antibodies; ns, not significant.

Ann Lab Med 2024;44:545~552 https://doi.org/10.3343/alm.2024.0132