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Table. 1. MRM transitions and other mass spectrometric settings
Analyte Q1 (m/z) Q3 (m/z) DP (V) EP (V) CE (V) CXP (V) CUR (psi) CAD (psi) Temp (°C) Dwell time (msec)
C-peptide1 (quantifier) 1,064.262 Da 171.2 Da 71 10 169 12 40 11 400 150
C-peptide2 (qualifier) 1,064.262 Da 955.2 Da 160 10 40 25 40 11 400 150
C-peptide IS (D8-Val7,10 C-peptide) 1,069.915 Da 171.2 Da 76 10 159 16 40 11 400 300

Abbreviations: MRM, multiple reaction monitoring; IS, internal standard; DP, de-clustering potential; EP, exit potential; CE, collision energy; CXP, collision cell exit potential; CUR, curtain gas, CAD, collision gas; Temp, temperature.

Ann Lab Med 2025;45:62~69 https://doi.org/10.3343/alm.2024.0072

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