Analyte | Q1 (m/z) | Q3 (m/z) | DP (V) | EP (V) | CE (V) | CXP (V) | CUR (psi) | CAD (psi) | Temp (°C) | Dwell time (msec) |
---|---|---|---|---|---|---|---|---|---|---|
C-peptide1 (quantifier) | 1,064.262 Da | 171.2 Da | 71 | 10 | 169 | 12 | 40 | 11 | 400 | 150 |
C-peptide2 (qualifier) | 1,064.262 Da | 955.2 Da | 160 | 10 | 40 | 25 | 40 | 11 | 400 | 150 |
C-peptide IS (D8-Val7,10 C-peptide) | 1,069.915 Da | 171.2 Da | 76 | 10 | 159 | 16 | 40 | 11 | 400 | 300 |
Abbreviations: MRM, multiple reaction monitoring; IS, internal standard; DP, de-clustering potential; EP, exit potential; CE, collision energy; CXP, collision cell exit potential; CUR, curtain gas, CAD, collision gas; Temp, temperature.
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