pISSN 2234-3806
eISSN 2234-3814

Table. 1.

Table 1 Allele-specific PCR and sequencing primers used in this study

Method	Target region	Forward primer sequence	Reverse primer sequence	Product size (bp)
Allele-specific PCR	RHD promotor	TCCACTTTCCACCTCCCTGC	GCAGCCAACTTCCCCTGTG	256
	RHD intron 4	CCTATTTTGGGCTGTCTGTGG	GAACCTGCTCTGTGAAGTGCT	298
	RHD exon 7	GTTGTAACCGAGTGCTGGGGATTC	TGCCGGCTCCGACGGTATC	123
	RHD exon 10	GATTTTAAGCAAAAGCATCCAAG	ATGGTGAGATTCTCCTCAAAGAG	191
	HBB*	GAAGAGCCAAGGACAGGTAC	GGAAAATAGACCAATAGGCAG	408
Sequencing	RHD exon 9	GGTCCAGGAATGACAGGGCT	GTTTCTTCCAGCTTTTGCATTGT	589

*The β-globin (HBB) housekeeping gene was used as the reference.