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Molecular Typing of Staphylococcus aureus Isolated from Blood on the Basis of Coagulase Gene Polymorphism and Toxin Genes
2008; 28(4): 286-292
Article
Brief Communication
Ann Lab Med 2015; 35(4): 454-457
Published online July 1, 2015 https://doi.org/10.3343/alm.2015.35.4.454
Copyright © Korean Society for Laboratory Medicine.
Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests
Sandrine Heraud, Pharm.D.1, Anne-Marie Freydiere, Pharm.D.1,2,3,4, Anne Doleans-Jordheim, Ph.D.1,5,
Michèle Bes, Ph.D. 1,2,3, Anne Tristan, Ph.D.1,2,3,4, François Vandenesch, M.D.1,2,3,4, Frederic Laurent, Ph.D.2,3,4,6,*,and Olivier Dauwalder, Ph.D.1,2,3,4,*
Hospices Civils de Lyon1, Laboratoire de Bactériologie, Centre de Biologie et de Pathologie Est, Bron; Hospices Civils de Lyon2, Centre National de Référence des Staphylocoques, Centre de Biologie et de Pathologie Est, Bron; International Center for Research in Infectiology3, INSERM U 1111, Lyon; Université de Lyon4, Faculté de Médecine Lyon Est, Domaine de la Buire, Lyon; Research group on «Bacterial Opportunistic Pathogens and Environment»5, UMR 5557 Ecologie Microbienne, CNRS, Université Lyon 1, ENVL, Université de Lyon, Lyon; Hospices Civils de Lyon6, Laboratoire de Bactériologie, Centre de Biologie et Pathologie Nord, Lyon, France
Correspondence to: Olivier Dauwalder
INSERM U1111-Pathogénie Bactérienne et Immunité Innée-Faculté de Médecine Lyon Est-Domaine de la Buire 7, rue Guillaume Paradin, 69008 Lyon, France
Tel: +33-0472-071-839
Fax: +33-0472-071-842
E-mail: olivier.dauwalder@chu-lyon.fr
*Olivier Dauwalder and Frederic Laurent shared in the supervision of this study.
Received: September 15, 2014; Revised: January 6, 2015; Accepted: April 25, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
Keywords: Staphylococcus aureus, Methicillin resistance, Blood culture, Accuracy, Immunochromatographic test