Article
Original Article
Korean J Lab Med 2009; 29(4): 338-344
Published online August 1, 2009 https://doi.org/10.3343/kjlm.2009.29.4.338
Copyright © Korean Society for Laboratory Medicine.
Anti-Inflammatory Effect of Near-Infrared Irradiated Cell Culture Media
Sang-Gyung Kim, M.D.1, Im-Hee Shin, Ph.D.2, Chang-Hyuk Choi, M.D.3, and Jung-Yoon Choe, M.D.4
Departments of Laboratory Medicine1, Medical Statistics2, Orthopedic Surgery3, and Internal Medicine4, Catholic University of Daegu, School of Medicine, Daegu, Korea
Correspondence to: Sang-Gyung Kim, M.D.
Department of Laboratory Medicine, Catholic University of Daegu, School of Medicine, 3056-6 Daemyeong 4-dong, Nam-gu, Daegu 705-718, Korea
Tel : +82-53-650-4145, Fax : +82-53-653-8672
E-mail : sgkim@cu.ac.kr
*본 연구는 경북대학교 휴먼팩트연구소의 지원과 2006년도 대구가톨릭대학교 의과학연구소 연구비의 지원으로 이루어졌음.
Background : Near-infrared light (NIR, 0.8-1.5 μm light) has been used in therapeutic devices for various injuries such as infected, ischemic and hypoxic wound. NIR-emitting technology has been developed recently in Korea. We hypothesized that NIR may have an anti-inflammatory effect and investigated the effect of NIR-irradiated media on cell culture.
Methods : Three kinds of cell lines, CAPE (vascular endothelial cell), NIH3T3 (fibroblast), and RD (smooth muscle cell) cells were cultured for 4 days in 10% FBS-containing media (1×104 cells/well), which were irradiated or not irradiated (control) by Eco-NFIR Drive (Model #0210, Ecowavetech, Korea). The cells were stimulated by 10 mcg/mL of bacterial lipopolysaccharide (LPS) or sodium nitroprusside (SNP). Cellular proliferation was measured by methylthiazol tetrazolium assay. Expression of interleukin (IL)-1 beta and nitric oxide was measured by ELISA. Expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was measured by immunofluorescence staining.
Results : NIR-irradiated medium was favorable for CAPE cell proliferation (N=8, P=0.000). IL-1 beta secretion from LPS-stimulated NIH3T3 cells incubated in the NIR medium was below that of control medium (N=4, P=0.026). Nitrate production seemed to be low in NIR-irradiated medium although statistically insignificant (N=4, P=0.076). Expression of iNOS of the LPS-stimulated cells was decreased in NIR medium, however, Cox-2 expression was not different between the two media.
Conclusions : NIR-irradiated medium supported vascular endothelial cell proliferation and showed an anti-inflammatory effect on fibroblast culture. These results can be used as basic data for future research on the clinical application of NIR.
Keywords: Near-infrared (NIR), Anti-inflammatory mediator, IL-1 beta, Nitric oxide (NO), Inducible nitric oxide synthase (iNOS)