Letter to the Editor
Ann Lab Med 2020; 40(4): 337-340
Published online July 1, 2020 https://doi.org/10.3343/alm.2020.40.4.337
Copyright © Korean Society for Laboratory Medicine.
First Korean Case of Partial D DBS-1
1Department of Laboratory Medicine, Soonchunhyang University Hospital Cheonan, Soonchunhyang University College of Medicine, Cheonan, Korea; 2Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul, Korea; 3Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Correspondence to: Duck Cho, M.D.
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: +82-2-3410-2403, Fax: +82-2-3410-2719, E-mail: firstname.lastname@example.org
*These authors equally contributed to this study.
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Partial D phenotypes have historically been classified using epitope studies  and, recently, using genetic studies. DBS is a partial D phenotype characterized by c.[676G>C]+[697G>C] (NM_016124.4) and has been named based on its positive reactivity with the D monoclonal antibodies (MoAbs) BS228 and BS233 (Biotest, Dreieich, Germany) . Three DBS subtypes have been reported till date:
The proband was a Korean woman with fibrocystic breast changes, who was admitted to the Samsung Medical Center. D typing using anti-D Bioclone (MAD2 clone; Ortho Clinical Diagnostics, Raritan, NJ, USA) was negative. Weak D testing using anti-D Bioclone and human IgG/IgM monoclonal anti-D (Millipore, Livingston, UK) yielded a result of grade 2+, while the result of partial D testing using D-screen (Diagast, Loos, France) was consistent with
The RhCE phenotype was ccEe (anti-C, -c, -E, and -e antibodies were obtained from Bioclone, Ortho Clinical Diagnostics, Buckinghamshire, UK), and
In various partial D phenotypes, such as DIIIa, DVa, DVI, DAR, DFR, DBT, and DBS,
The genetic basis of the RhD blood group differs across races and ethnicities. For example, the RhD-negative phenotype mainly results from
In conclusion, we reported the first case of
Results of the genetic analysis of the proband and her family members. (A) Long-range PCR with primers located in non-Rhesus box sequences. A 2,778-bp fragment was amplified by PCR, indicating the presence of a hybrid
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