Article

Letter to the Editor

Ann Lab Med 2020; 40(5): 424-427

Published online September 1, 2020 https://doi.org/10.3343/alm.2020.40.5.424

Copyright © Korean Society for Laboratory Medicine.

False Positive Class II HLA Antibody Reaction Due to Antibodies Against Denatured HLA Might Differ Between Assays: One Lambda vs. Immucor

Borae G. Park, M.D., Ph.D.1 , Younhee Park, M.D., Ph.D.2 , Beom Seok Kim, M.D., Ph.D.3 , Yu-Seun Kim, M.D., Ph.D.4 and Hyon-Suk Kim, M.D., Ph.D.2

1Department of Laboratory Medicine, Korea University Guro Hospital, Seoul, Korea; Departments of 2Laboratory Medicine, 3Nephrology, and 4Transplant Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea

Correspondence to: Younhee Park, M.D., Ph.D.
Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea
Tel: +82-2-2228-2440, Fax: +82-2-364-1583, E-mail: younheep@gmail.com

Received: October 6, 2019; Revised: January 3, 2020; Accepted: March 13, 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fig. 1.

Reactivity patterns of class II HLA antibody assays. (A) LABScreen SAB class II (One Lambda, Canoga Park, LA, USA) SABA showing pan-HLA-DR positivity. Self-HLA-DR antigens are indicated by red circles. (B) Lifecodes class II SABA (Immucor, Stamford, CT, USA) showing positivity for HLA-DR51 and -DR9. (C) PRA Identification Bead Reaction (LABScreen PRA, One Lambda) showing weak positivity for HLA-DR51, DR15, and DR16.

Abbreviations: see Table 1.


Assignment of class II HLA antibody specificity according to bead type and manufacturer

Kit usedClass II HLA antibody specificity identified in each essay*Source of antigen
One Lambda SABA (LABScreen SAB)Strong:_DR1, DR4, DR7, DR8, DR9, DR10, DR11, DR12, DR13, DR14, DR15, DR16, DR17, DR18, DR51, DR52, DR52, DR103, DQ2, DQ4, DQ5, DQ6, DQA1*01:01, DQA1*01:02, DQA1*03:01, DQA1*04:01
Moderate: DQ8, DQ9
Weak: DQA1*02:01
Recombinant HLA molecule
Immucor SABA (Lifecodes LSA)Weak: DR51, DR103, DR9Recombinant HLA molecule
One Lambda screening (LABscreen Mixed)Negative for class II HLA antibodiesPurified human HLA
One Lambda identification (LABscreen PRA)Weak: DR15, DR16, DR51Purified human HLA

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