Letter to the Editor
Ann Lab Med 2021; 41(4): 436-438
Published online July 1, 2021 https://doi.org/10.3343/alm.2021.41.4.436
Copyright © Korean Society for Laboratory Medicine.
First Identification of IMP-1 Metallo-β-Lactamase in Delftia tsuruhatensis Strain CRS1243 Isolated From a Clinical Specimen
Sun-Mi Cho, M.D.1 , Seong Geun Hong, M.D., Ph.D.1 , Yangsoon Lee, M.D., Ph.D.2 , Wonkeun Song, M.D., Ph.D.3 , Dongeun Yong, M.D., Ph.D.4 , Seok Hoon Jeong, M.D., Ph.D.4 , Kyungwon Lee, M.D., Ph.D.4 , and Yunsop Chong, Ph.D.4
1Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea; 2Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea; 3Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea; 4Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea
Correspondence to: Seong Geun Hong, M.D., Ph.D.
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 13496, Korea
Tel: +82-31-780-5463, Fax: +82-31-780-5476
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The isolate was suspected to produce carbapenemases based on routine antimicrobial susceptibility testing using Vitek AST N212 cards (bioMerieux). Antimicrobial susceptibility was determined according to the CLSI Minimum Inhibitory Concentration (MIC) Interpretive Standards for other non-
Multiplex PCR was performed to detect the specific MBL. The specific primer pairs for the detection of the New Delhi MBL and Sao Paulo MBL were designed in this study and those for the detection of Verona integron-encoded MBL, imipenemase (IMP), Seoul IMP, and German IMP were selected from a previous report (Table 1). The sequence of the bacterial integron carrying the MBL-encoding gene was identified using primer walking (Table 1). We found that
Figure 1. Schematic representation of the 8,846-bp partial DNA sequence of the class 1 integron containing
blaIMP-1 within a Tn 402-like module in Delftia tsuruhatensisCRS1243.
Cases of human infection with
To the best of our knowledge, this is the first report of IMP-1 MBL production from a
Hong SG conceptualized and designed the study and coordinated the drafting of the manuscript. Hong SG performed the microbiologic test and PCR work. Hong SG, Cho SM, and Lee Y performed data analysis and wrote the manuscript. Song W, Yong D, Jeong SH, Lee K, and Chong Y supervised the study, reviewed and commented on the manuscript, and approved the final draft. All authors read and approved the final manuscript.
CONFLICTS OF INTEREST
The authors declare no conflicts of interest.
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