Article

Original Article

Korean J Clin Pathol 2001; 21(1): 67-71

Published online February 1, 2001

Copyright © Korean Society for Laboratory Medicine.

Evaluation of LG Malaria Anti-Pv for Diagnosis of Plsamodium vivax Malaria in the Repub1ic of Korea

정준용 · 김명유 · 임국진 · 손미진 · 고원규

Abstract

Background :In the Republic of Korea, Plasmodium vivax, which had disappeared since 1984, re-emerged in 1993. Currently, malaria is becoming a serious public health problem in the Republic of Korea. The diagnosis of malaria has relied on microscopic examination such as thin and thick blood smears. However, even for expert microscopists, this test is a laborious and time-consuming procedure. Therefore, the development of a reliable, easy, and convenient diagnostic test is crucial. Recently, the LG malaria anti-Pv enzyme-linked immunosorbent assay (ELISA) kit for the detection of a specific antibody against the merozoite surface protein (MSP) of P. vivax was developed. The aim of this study was to evaluate the diagnostic kit for P. vivax malaria in the Republic of Korea. Method :To determine the usefulness of the LG malaria anti-Pv as a diagnostic kit for vivax malaria, a total of 59 serum samples from patients with P. vivax malaria were tested. The patients were diagnosed microscopically and the parasitemia index of their blood was calculated. Sera from 203 uninfected healthy blood donors, which were microscopically negative for Plasmodium vivax were used as negative controls. Result :The sensitivity and specificity of the LG malaria anti-Pv were 98,31% (58/59) and 98.03% (199/203), respectively. The false-positive and false-negative rates were 1.97% (4/203) and 1.69% (1/59), respectively. Conclusion :The diagnostic kit, LG malaria anti-Pv, might be a useful tool for diagnosis and screening of P. vivax malaria in Korea.

Keywords: Malaria, Plasmodium vivax, Diagnosis, Enzyme-linked immunosorbent assay, Korea