Case Report

Korean J Lab Med 2009; 29(4): 277-281

Published online August 1, 2009

Copyright © Korean Society for Laboratory Medicine.

Clinical Utility of Chimerism Status Assessed by Lineage-Specific Short Tandem Repeat Analysis: Experience from Four Cases of Allogeneic Stem Cell Transplantation

Ri-Young Goh, M.S.1, Sung-Suk Cho, M.T.1, Yoo-Jeong Song, M.S.1, Kyeong Heo, M.S.1, Sung-Yong Oh, M.D.2, Sung-Hyun Kim, M.D.2, Hyeok-Chan Kwon, M.D.2, Hyo-Jin Kim, M.D.2, and Jin-Yeong Han, M.D.1

Departments of Laboratory Medicine1 and Internal Medicine2, Dong-A University College of Medicine, Busan, Korea

Correspondence to: Jin-Yeong Han, M.D.
Department of Laboratory Medicine, Dong-A University College of Medicine, 1 Dongdaesin-dong 3-ga, Seo-gu, Busan 602-715, Korea
Tel : +82-51-240-5323, Fax : +82-51-255-9366
E-mail :

*This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A050099).

Received: November 10, 2008; Revised: May 24, 2009; Accepted: July 20, 2009


Chimerism testing permits early prediction and documentation of successful engraftment, and also facilitates detection of impending graft rejection. In this study, we serially monitored chimerism status by short tandem repeat-based PCR in nucleated cells (NC), T cells and natural killer (NK) cells after myeloablative allogeneic stem cell transplantation (SCT). Four patients with myeloid malignancies
showed discrepant chimerism results among those three fractions. Three patients had mixed chimerism (MC) of donor/host T cells at a time point around the onset of chronic graft-versus-host disease (GVHD). In two patients with disease relapse, MC of NK cells preceded a morphological relapse or NK cells showed a higher percentage of patient cells compared to NC. Therefore, our study shows that chimerism analysis in lineage-specific cells might be useful in predicting clinical outcome after allogeneic SCT in certain patients. 

Keywords: Lineage-specific chimerism, Myeloablative stem cell transplantation, Myeloid malignancy, T cells, NK cells