Manufacturing Cell and Gene Therapies: Challenges in Clinical Translation
2024; 44(4): 314-323
Korean J Lab Med 2011; 31(3): 212-218
Published online July 1, 2011 https://doi.org/10.3343/kjlm.2011.31.3.212
Copyright © Korean Society for Laboratory Medicine.
Hee Soon Cho, M.D.1, In Hwan Song, M.D.2, So-Young Park, M.D.3, Min Cheol Sung, M.D.4, Myun-Whan Ahn, M.D.4, and Kyung Eun Song, M.D.5
Departments of Laboratory Medicine1, Anatomy2, Physiology3, and Orthopedic Surgery4, College of Medicine, Yeungnam University, Daegu;Department of Clinical Pathology5, Kyungpook National University School of Medicine, Daegu, Korea
Correspondence to: Myun-Whan Ahn, M.D.
Department of Orthopedic Surgery, Yeungnam University College of Medicine, 317-1 Daemyeong-dong, Nam-gu, Daegu 705-717, Korea
Tel: +82-53-620-3643, Fax: +82-53-628-4020, E-mail: mwahn@ynu.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs).
Methods: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured.
Results: The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-β1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration.
Conclusions: The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.
Keywords: Platelet-rich plasma, Mesenchymal stem cells, Growth factors, Transforming growth factor-β1, Platelet-derived growth factor-BB