Article

Original Article

Ann Lab Med 2015; 35(4): 423-428

Published online July 1, 2015 https://doi.org/10.3343/alm.2015.35.4.423

Copyright © Korean Society for Laboratory Medicine.

Comparison of FcRγ-Deficient and CD57+ Natural Killer Cells Between Cord Blood and Adult Blood in the Cytomegalovirus-Endemic Korean Population

Hee Jo Baek, M.D.1, Da-Woon Kim, M.D.2,3, Minh-Trang Thi Phan, M.S.2,4,5, Ju-Sun Kim, M.S.5, Ji-Hoon Yang, M.S.6, Jeong Il Choi, M.D.6, Je-Jung Lee, M.D.5, Myung-Geun Shin, M.D.2, Dong-Wook Ryang, M.D.2, Sang-Ki Kim, D.V.M.7, Seung-Hwan Lee, Ph.D.8, Hoon Kook, M.D.1, and Duck Cho, M.D.2,4,5,*

Department of Pediatrics1, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine2, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine3, KS Hospital, Gwangju; Center for Creative Biomedical Scientists4, Chonnam National University, Gwangju; Research Center for Cancer Immunotherapy5, Chonnam National University Hwasun Hospital, Hwasun; Department of Anesthesiology and Pain Medicine6, Chonnan National University Medical School and Hospital, Gwangju; Department of Companion & Laboratory Animal Science7, Kongju National University, Yesan, Korea; Department of Biochemistry, Microbiology and Immunology8, University of Ottawa, Ottawa, Canada

Correspondence to: Duck Cho
Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, 322 Seoyang-ro, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Korea
Tel: +82-61-379-7951
Fax: +82-61-379-7984
E-mail: hasomii@hanmail.net
*Current address: Deparment of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 135-710, Korea
Tel: +82-2-3410-2403
Fax: +82-2-3410-2719
E-mail: duck.cho@samsung.com
Hoon Kook
Department of Pediatrics, Chonnam National University Hwasun Hospital, 322 Seoyang-ro, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Korea
Tel: +82-61-379-7696
Fax: +82-61-379-7697
E-mail: hoonkook@chonnam.ac.kr

Received: August 24, 2014; Revised: December 3, 2014; Accepted: April 2, 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Background: FcRγ-deficient natural killer (NK) cells (g¯NK cells) have been associated with cytomegalovirus (CMV) infection. However, the frequency of g¯NK cells in a CMV-endemic area (i.e., Korea) has not yet been studied. We examined the frequency of g¯NK cells and expression of CD57 on NK cells in cord blood (CB) and adult blood (AB).
Methods: Of the 24 AB samples collected, 95.8% (23/24) were CMV IgG+/IgM-, while 100% of the 13 healthy CB samples were CMV IgG+/IgM-. We performed whole-blood flow cytometry assays to analyze intracellular FcRγ and CD3ζ expression of CD3-/CD56dim NK cells from 13 CB and 24 AB samples, and surface CD57 expression on CD3-/CD56dim/CD16+ NK cells from 13 CB and 19 AB samples.
Results: All CMV seropositive AB samples contained g¯NK cells (23/23), and the median proportion of g¯NK cells in the CD3-/CD56dim NK cell pool was 35.0% (range: 11-77%). CD57+ NK cells in the CD3-/CD56dim/CD16+ NK cell population were detected in all 19 AB samples tested, but not in any CB samples.
Conclusions: Our data suggest that g¯NK cells and CD57+ NK cells are present at a very high frequency in CMV-seropositive AB, but rare in CMV-naïve CB.

Keywords: Adult, Blood, Cord, Cytomegalovirus, Infection, FcRγ, Natural killer cell

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