Article

Review Article

Ann Lab Med 2016; 36(3): 203-208

Published online May 1, 2016 https://doi.org/10.3343/alm.2016.36.3.203

Copyright © Korean Society for Laboratory Medicine.

Korean Society for Laboratory Medicine Practice Guidelines for the Molecular Diagnosis of Middle East Respiratory Syndrome During an Outbreak in Korea in 2015

Chang-Seok Ki, M.D.1,*, Hyukmin Lee, M.D.2,*, Heungsup Sung, M.D.3, Sinyoung Kim, M.D.4, Moon-Woo Seong, M.D.5, Dongeun Yong, M.D.4, Jae-Seok Kim, M.D.6, Mi-Kyung Lee, M.D.7, Mi-Na Kim, M.D.3, Jong-Rak Choi, M.D.4, Jeong-Ho Kim, M.D.4, and The Korean Society for Laboratory Medicine MERS-CoV Laboratory Response Task Force

Department of Laboratory Medicine and Genetics1, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul; Department of Laboratory Medicine2, Catholic Kwandong University College of Medicine, Incheon; Department of Laboratory Medicine3, University of Ulsan College of Medicine and Asan Medical Center, Seoul; Department of Laboratory Medicine4, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine5, Seoul National University College of Medicine, Seoul; Department of Laboratory Medicine6, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul; Department of Laboratory Medicine7, Chung-Ang University College of Medicine, Seoul, Korea

Correspondence to: Mi-Na Kim
Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
Tel: +82-2-3010-4511
Fax: +82-2-478-0884
E-mail: mnkim@amc.seoul.kr

*Chang-Seok Ki and Hyukmin Lee equally contributed as first authors.

This KSLM guideline was the part of the official MERS practice guideline of Central MERS-CoV Control Office published online through the homepage of The Korean Society of Infectious Diseases (http://www.ksid.or.kr/) during the outbreak.

Received: August 31, 2015; Revised: November 13, 2015; Accepted: January 29, 2016

Abstract

For two months between May and July 2015, a nationwide outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) occurred in Korea. On June 3, 2015, the Korean Society for Laboratory Medicine (KSLM) launched a MERS-CoV Laboratory Response Task Force (LR-TF) to facilitate clinical laboratories to set up the diagnosis of MERS-CoV infection. Based on the WHO interim recommendations, the Centers for Disease Control and Prevention of United States guidelines for MERS-CoV laboratory testing, and other available resources, the KSLM MERS-CoV LR-TF provided the first version of the laboratory practice guidelines for the molecular diagnosis of MERS-CoV to the clinical laboratories on June 12, 2015. The guidelines described here are an updated version that includes case definition, indications for testing, specimen type and protocols for specimen collection, specimen packing and transport, specimen handling and nucleic acid extraction, molecular detection of MERS-CoV, interpretation of results and reporting, and laboratory safety. The KSLM guidelines mainly focus on the molecular diagnosis of MERS-CoV, reflecting the unique situation in Korea and the state of knowledge at the time of publication.

Keywords: Guidelines, Middle East respiratory syndrome coronavirus, Molecular diagnosis, Outbreak